The dual beam UV visible spectrophotometer is a high-precision analytical instrument widely used in fields such as chemistry, biology, medicine, and materials science. Its core advantage lies in the ability to simultaneously measure signals from both the sample cell and the reference cell, effectively eliminating the effects of light source fluctuations and environmental interference, and improving measurement accuracy and stability. The following are its main application scenarios and corresponding testing types:

1、 Basic quantitative analysis

1. Determination of concentration by standard curve method

Principle: According to Lambert Beer's law, the concentration of an unknown sample can be inverted by drawing a standard curve of a known concentration series.

Advantage: The dual beam design can deduct blank background in real time, reducing the impact of baseline drift on low concentration samples.

2. Dynamics research (reaction rate monitoring)

Applicable scenarios: Track the absorbance changes of chemical reactions over time, calculate reaction order, activation energy and other parameters.

Features: The fast continuous scanning function supports data acquisition with millisecond level time resolution.

2、 Qualitative and structural identification of substances using a dual beam UV visible spectrophotometer

1. Identification of characteristic absorption peaks

Function: Determine functional groups or molecular structures based on the position and intensity of absorption peaks at specific wavelengths.

Analysis of Conjugated Organic Systems: Strong Absorption Characteristics of Conjugated Double Bonds, Aromatic Rings, etc. in the UV Region;

Protein secondary structure prediction: Circular dichroism signal in the far ultraviolet region (<250nm) assists in α/β conformation analysis;

Nucleic acid purity assessment: The A260/A280 ratio reflects the degree of protein impurity contamination in DNA/RNA samples.

Tip: Combining derivative spectroscopy technology to analyze overlapping peaks and enhance the ability to distinguish components in complex mixtures.

2. Spectral matching library search

Database comparison: Match the measured spectra with standard spectral libraries (such as SDBS, NIST) to quickly identify compound identities.

3、 Advanced Material Characterization

1. Research on Optical Properties of Nanomaterials

Key indicators: Surface Plasmon Resonance (SPR), absorption sideband shift caused by quantum size effect.

Analysis of Local Field Enhancement Effect of Gold/Silver Nanoparticles;

Optimization of exciton absorption peak regulation in quantum dots.

Pattern innovation: Study on temperature dependent behavior under the linkage of variable temperature experimental devices.

2. Performance evaluation of pigments/dyes

Test dimension:

Light fastness test (absorbance attenuation curve before and after accelerated aging);

Quantification of hair color intensity and color stability;

Analysis of differences in aggregation behavior in different solvent systems.

Industry value: Guiding the setting of textile printing and dyeing process parameters, optimizing ink formula stability.

4、 Analysis of Interactions between Biomacromolecules Using Dual Beam UV Visible Spectrophotometer

1. Protein ligand binding experiment

Methodological support: Titration method is used to determine the binding constant KD and stoichiometric ratio n.

Example: Affinity screening of drug small molecules and receptor targets;

Signal amplification strategy: Adopt competitive permutation method to improve detection sensitivity to nM level.

Instrument configuration expansion: Optional constant temperature circulating water bath system to maintain physiological temperature conditions (37 ℃).

2. Validation of nucleic acid hybridization efficiency

Application scenarios: PCR product quantification, probe labeling efficiency evaluation.

Technical point: Utilizing the fluorescence quenching effect of fluorescent labeled primers to achieve high-sensitivity detection.