1 Overview

QX-II Anaerobic incubatorIt is a device for bacterial cultivation and operation under low oxygen environmental conditions. It can provide strict anaerobic conditions with constant temperature cultivation and has a systematic and scientific working area. Operating and cultivating in this device can cultivate anaerobic organisms that are difficult to grow, while avoiding the risk of anaerobic organisms dying due to exposure to oxygen during previous operations in the atmosphere. Therefore, this device is an ideal tool for anaerobic biological detection research. This device is also a versatile instrument that provides favorable growth conditions for the growth and reproduction of microaerophilic bacteria by changing the operating mode.

II QX-Characteristics of Anaerobic Incubator II

QX-II Anaerobic incubatorIt consists of a vacuum sampling chamber, a constant temperature anaerobic operating chamber, an air circuit, a circuit control system, and other components. The whole machine has a novel design and compact structure, with advantages such as good anaerobic environment, good air tightness, precise temperature control, good stability, easy use, gas saving, economy, and safe and reliable operation. Its characteristics are as follows:

1. Using scientific methods to achieve precision in anaerobic environments, it has good constant performance and reliable use;

2. The temperature of the incubator is controlled by a microcomputer, which can accurately and intuitively reflect the temperature inside the incubator. It can automatically control the temperature again and is an effective temperature limiting protection device. The culture grows under safe temperature conditions;

3. The box is equipped with ultraviolet sterilization lamps, which can effectively prevent bacterial contamination;

4. Gas path device, capable of accurately adjusting flow rate and inputting various required gases at will;

5. The pneumatic circuit is controlled by a microcomputer, and the solenoid valve can be started or closed with flexible operation;

6. The front window of the operating room is made of thick transparent glass, which allows for clear and direct observation of indoor operations. The use of plastic gloves is reliable, comfortable, flexible, and easy to use;

7. There is a deoxygenation catalyst installed indoors.

III QX-II. Main technical indicators of anaerobic incubator

1. The anaerobic state in the sampling room takes less than 5 minutes to form;

2. The anaerobic formation time in the operating room is less than 1 hour (pumping and inflation replacement);

3. The anaerobic environment is maintained for more than 10 hours in the operating room when the replenishment of trace mixed gases is stopped;

4. Operating temperature: room temperature+3℃~50℃；

5. Temperature fluctuation in the cultivation room ≤± 0.3℃Temperature distribution uniformity ≤± 1.0℃；

6. Operating room volume: 820mm × 550mm × 660mm (length × width),

7. Incubator size: 250mm × 190mm × 290mm

8. power supply220V±10%AC, Power:500VA
4QX-II Anaerobic incubatorinstall

1. The whole machine should be placed in a location with a small temperature difference and easy operation, avoiding direct sunlight and being away from heating equipment, and placed steadily;

2. Place the mixed gas cylinder and nitrogen cylinder steadily, and install pressure reducing valves (including pressure gauges) in appropriate positions;

3. Connect the air circuit and check it. To prevent air leakage, if necessary, use sealant to bond at each connection point;

5 QX-II. Use of Anaerobic Incubator

（1） Formation of anaerobic environment in the operating room:

1. Place necessary accessories and tools according to usage requirements;

2. Turn on the power, turn on the lighting, turn on the temperature controller, and adjust the desired temperature;

3. Place 1000g of palladium granules (sealed) and 500g of desiccant in the operating room, and put in Meilan indicator paper (sealed);

4. Close the indoor and outdoor doors of the sampling room tightly and perform vacuum calibration;

5. Number of replacements in the operating room (nitrogen replacement):

A. Put latex gloves on the flange ring of the observation board and tighten them tightly

B. Connect the nitrogen intake path, open nitrogen control valve 1, make the gloves bulge, close valve 1, and then tighten the bag opening;

6. Repeat the nitrogen filling process for the second replacement of the operating room (nitrogen replacement), evacuate the sampling room first, and pay attention to using the foot switch to open and close the exhaust at any time. Three consecutive nitrogen replacements.

7. The fourth replacement of the operating room (mixed gas replacement):

The mixed gas ratio is N₂ 85%、H₂ 10%、CO₂ 5%）

A. Switch the air circuit and open the mixing valve 3 for intake. When inflating, evacuate the sampling chamber first and use the foot switch to open and close the exhaust at any time;

B. Close the mixed gas valve 3 and open valve 5 to allow the mixed gas to pass through the flow meter. Adjust the flow meter to a flow rate of approximately 10ml per minute;

C. The mixed gas is repeatedly converted 2-3 times, and through the above process, an anaerobic environment is basically formed.

8. Turn on the granular deoxygenation agent in the operating room, connect the power supply of the deoxygenation catalyst for catalytic deoxygenation, and after one hour, open the Meilan indicator paper to observe its color change. If it does not change color, it means that the operating room has reached an anaerobic environment;

9. Turn on the ultraviolet sterilization lamp and sterilize indoors. The sterilization time is determined according to the specific experiment.

（2） Placement and cultivation of bacterial strains:

1. Check the door inside the sampling room and close it tightly;

2. Open the outdoor door of the sampling room, place the bacterial strain into the sampling room, and then close the door;

3. The process of nitrogen replacement in the sampling room is as follows: turn on the vacuum pump, first evacuate to a vacuum degree of 500ml mercury column (66kPa) or above, stop, then manually open nitrogen valve 2 for air intake, make the pointer return to zero position, and close valve 2. Repeat the operation once for the second time. During the third operation, stop at a vacuum level of 500ml mercury (66kPa) or higher, then open valve 4 to introduce mixed gas and return the pointer to zero position, and close valve 4. The three nitrogen replacement processes in the sampling room have been completed;

4. If a lower vacuum degree is selected, the number of replacements needs to be increased;

5. Open and close the outdoor door for sampling, and then lower the vacuum level by 100ml of mercury (13kPa) for testing;

6. Conditions for long-term continuous use of anaerobic incubators:

A. Open the Meilan indicator paper in the operating room every day to observe. If it is not normal, it must be ventilated again;

B. Long term continuous input of trace amounts of mixed gas is required, so that the added nitrogen can combine with trace amounts of oxygen through catalytic absorption. In an anaerobic indoor state, the flow rate of the added mixed gas is selected to be around 10ml per minute;

C. Continuously cultivate and run for one day, and replace the deaerator and desiccant once.

D. The temperature inside the operating room can be freely selected and controlled.

7. Adjustment of output pressure for mixed gas cylinders and nitrogen cylinders: Adjust the pressure reducing valve to achieve an output pressure of around 0.1Mpa.

VI Precautions

1. Instruments should be installed in places with clean air and minimal temperature changes as much as possible;

2. Before starting up, it is necessary to fully familiarize and understand the instructions for using the various components and supporting instruments, and master the correct usage methods;

3. The culture must be placed after reaching an anaerobic environment in the operating room;

4. If a malfunction occurs (due to gas outage or other reasons), the operating room can still maintain anaerobic state for 10 hours (if it exceeds 10 hours, the culture can be taken out for further processing as needed);

5. Regularly pay attention to low air leakage in the air circuit;

6. When replacing gas cylinders, be careful to tighten the trachea to avoid the flow of oxygen-containing gas;

Use the vacuum pump as required and regularly check and refuel it.