As a core equipment for cell culture, microbial fermentation, and enzyme reaction research, the precise environmental control and oscillation function of the light controlled constant temperature shaker are crucial for experimental results. This article is based on JingdaZQJD-180GZCO ₂Taking the CO2 illuminated constant temperature shaker as an example, this article systematically explains the usage method and key precautions to assist researchers in standardizing operations and improving experimental efficiency.

1、 Preparation before use: environmental inspection and parameter preset

Installation environment requirements

The equipment should be placed on a horizontal and well ventilated experimental platform, avoiding direct sunlight or proximity to heat sources.

Ensure that there are no strong electromagnetic interference sources (such as large motors and high-frequency equipment) around to prevent sensor signal distortion.

The power supply needs to be independently grounded, and the voltage fluctuation range should not exceed the rated value±10%.

Parameter preset

Temperature setting: Adjust the target temperature according to experimental requirements (such as37℃Used for mammalian cell culture, the system automatically starts heating by inputting numerical values through the touch screen/Refrigeration program.

CO₂Concentration setting: ConnectedCO₂After the gas source, set the concentration value (usually5%）Infrared sensors monitor and provide real-time feedback data to ensure stable gas inside the box.

Light and speed settings: Select the light intensity (such as5000LuxSimulate natural light and oscillation speed（100-200rpmCommonly used range), the three eccentric axis drive ensures synchronous oscillation of all samples.

2、 Operation process: from sample placement to data recording

Sample placement and fixation

Use specialized culture bottles or conical bottles to ensure good sealing of the bottle mouth and prevent leakage of the culture solution.

Place the sample evenly on the vibration table to avoid blocking the ventilation opening or light sensor.

The double-layer door design allows for observation of the sample with a semi open inner door, but it is recommended to control the entire operation time within1Reduce temperature fluctuations within minutes.

Operation monitoring and adjustment

After startup, pass throughLCDReal time temperature monitoring on the screenCO₂Concentration and speed curves, confirm that the parameters are stable within the set range.

If an alarm occurs (such as overheatingCO₂Immediately suspend operation and check the gas source, sensors, or ventilation system for abnormal concentration.

The timed defrosting function needs to be set at intervals based on the environmental humidity (such as4hour/To prevent the evaporator from freezing and affecting the refrigeration efficiency.

3、 Key precautions: equal emphasis on safety and maintenance

Safety operation standards

Do not move or tilt the device during operation to prevent liquid splashing and damaging the circuit.

replaceCO₂When using gas cylinders, it is necessary to close the gas source valve and wear protective gloves to prevent frostbite.

The UV sterilization function is only for use when no one is around, avoid direct exposure to the skin or eyes.

Key points of daily maintenance

Cleaning and disinfection: used weekly75%Wipe the inner container and countertop with alcohol, and activate ultraviolet sterilization every month30minute

Sensor calibration: Calibrate quarterly with standard gasCO₂Sensors to ensure accuracy±0.2%Within.

Motor maintenance: Check the lubrication of servo motor bearings annually and add high-temperature resistant grease if necessary.

Emergency handling of faults

No display or crash: Check the power connection. If the problem persists after restarting, contact the manufacturer for repair.

Excessive temperature fluctuations: Clean the condenser dust and check if the fan is running properly.

Abnormal oscillation: Adjust the balance of the three eccentric shafts or replace the worn drive belt.

Conclusion

The standardized use of a constant temperature shaker under illumination requires consideration of parameter control, operational safety, and regular maintenance. Through pre inspection, standardized processes, and preventive maintenance, the lifespan of equipment can be significantly extended and experimental repeatability can be ensured. Researchers should establish operation logs to record abnormal situations and their handling measures, providing a basis for subsequent experimental optimization. With the refined development of biotechnology, the efficient utilization of such equipment will become a key link in promoting scientific research innovation.