As a core equipment in microbial research, environmental monitoring, and biopharmaceutical fields, the precise temperature and humidity control ability of the mold biochemical incubator directly affects the reliability of experimental results. The Zhongwang Mold Biochemical Incubator has become the preferred solution for dynamic laboratory cultivation due to its intelligent design, high-precision sensing system, and multiple safety protection mechanisms. This article will systematically review its operation process and maintenance points to assist researchers in conducting experiments efficiently.

1、 Equipment installation and environmental configuration

Site selection requirements
The incubator should be placed on a stable and dry workbench, away from direct sunlight, electromagnetic interference sources, and strong vibration areas. The ambient temperature should be controlled between 5 ℃ and 40 ℃, the relative humidity should be ≤ 85%, and the ventilation should be good. For example, in microbial fermentation experiments, if the environmental humidity is too high, it may cause condensation inside the equipment, affecting the accuracy of the sensor.

Power supply and grounding
Use an AC 220V ± 22V power supply with a frequency of 50Hz ± 1Hz, and ensure reliable grounding of the equipment. The Zhongwang incubator is equipped with a dual fuse system (2A control power supply, 8A cooling and heating load), which can effectively prevent overload risks.

Initial cleaning and disinfection
first timeBefore use, wipe the inner walls and shelves with 75% alcohol to remove any production residues. When restarting after long-term disuse, it is necessary to check the water accumulation tray at the bottom of the box to avoid bacterial growth.

2、 Core parameter setting and regulation

temperature control

Setting method: Enter the parameter menu through the "SET" button on the panel, the main screen displays the measured values, and the secondary screen displays the set values. Press the "△/▽" key to adjust the target temperature (accuracy ± 0.1 ℃), covering a range of -10 ℃ to 80 ℃. For example, in the cultivation experiment of Aspergillus niger, the temperature needs to be stabilized at 28 ℃. At this time, the heating indicator light will light up and the system will automatically start the PID algorithm to maintain a constant temperature.

Alarm function: Press and hold the "SET" button for 3 seconds to set the upper limit (RL1) and lower limit (RL2) alarm values. When the temperature deviates from the set value by ± 1.5 ℃, the sound and light alarm system is activated to prevent the cultivation conditions from getting out of control.

Humidity regulation

Humidification system: Connect the independent humidifier to the back interface of the box, and add water to the water tank mark. Adjust the target value (range 30% -95% RH) through the humidity setting button, and the system will automatically start and stop the humidifier based on sensor feedback.

Dew condensation treatment: If the humidity sensor is in a high humidity environment for a long time, it should be turned off and the box door should be opened to dry naturally for 2 hours to avoid false high display values.

Oscillatory mode (optional)
Some models of Zhongwang support rotary (amplitude 50mm) or reciprocating oscillation, with a frequency adjustable to 500rpm. In cell suspension culture experiments, rotary oscillation can increase the density of HEK293 cells by 40%, while reciprocating oscillation is suitable for digesting adherent cells.

3、 Sample placement and cultivation process

Preprocessing stage

Use sterile culture dishes or test tubes to hold the culture medium, and sterilize at 121 ℃ for 20 minutes before inoculation.

Apply sterile cotton swabs evenly to mold spores (such as Penicillium and Aspergillus) to ensure consistent inoculation volume.

Sample placement specifications

The load capacity of the shelf should not exceed 80%, and the distance between items should be ≥ 5cm to avoid obstructing the convection of hot and cold air. For example, in the BOD measurement experiment, multiple water sample bottles need to be placed simultaneously, and a layered placement strategy should be adopted.

After closing the box door, check the integrity of the sealing strip to prevent temperature fluctuations.

Monitoring of the cultivation process

Record temperature and humidity data daily, and export historical curves through USB interface. Zhongwang incubator supports 100 sets of program storage and can preset gradient heating/cooling processes.

Regularly observe the growth of the samples, and if contamination is found, immediately terminate the experiment and disinfect the box.

4、 Safety operation and maintenance standards

Exception handling mechanism

When there is a power outage, immediately unplug the power and wait for 5 minutes before restarting to avoid frequent start stop damage to the compressor.

If the temperature control fails, check the back fuse and heating tube connection status, and if necessary, contact after-sales to replace the PT100 sensor.

Monthly maintenance plan

Cleaning: Wipe the inner wall with disinfectant and replace the distilled water in the water tray.

Lubrication: Add food grade grease to the reciprocating oscillation shaft to reduce mechanical wear.

Calibration: Contact the metrology department annually to verify the temperature sensor and ensure compliance with 21 CFR Part 11 specifications.

Long term suspension management

After power failure, empty the water tank and cover the equipment with a dust cover.

Power on for 1 hour every quarter to prevent the motor from getting damp and getting stuck.

5、 Typical application scenario cases

Optimization of antibiotic fermentation
A pharmaceutical company conducted a Streptomyces fermentation experiment using a Zhongwang incubator, simulating the in vivo fluid environment through biaxial composite oscillation. The soluble protein ratio was increased from 15% to 65%, significantly reducing downstream purification costs.

Intestinal organoid culture
In a three-dimensional cultivation system, the equipment maintains conditions of 37 ℃, 5% CO ₂, and 90% humidity, combined with microcarrier technology to achieve an organ formation efficiency of 85%, providing a standardized platform for disease model research.

Toxicity testing of industrial wastewater
By setting a gradient temperature of 15 ℃ -35 ℃ and simulating the water environment in different seasons, the inhibitory effect of heavy metals on activated sludge was evaluated, and the data repeatability CV value was less than 3%.

The Zhongwang Mold Biochemical Incubator covers diverse needs from basic research to industrial production through intelligent design and modular functions. Researchers need to strictly follow operating procedures, combined with regular maintenance, in order to fully utilize equipment performance and provide solid support for technological innovation in the field of life sciences.