Aspartyl phenylalanine methyl ester, commonly known as aspartame, with chemical formula of C14H18N2O5, is a natural functional oligosaccharide with high sweetness, no deliquescence and no caries. It is edible for diabetes patients. Aspartame, due to its extremely low calorie content and high sweetness, can be added to beverages, pharmaceuticals, or sugar free chewing gum as a sugar substitute. Normally, the sweetness of aspartame is 180-220 times that of sucrose. Aspartame, as an important sweetener, is widely used in pharmaceutical and food processing, and its safety is very important. However, aspartame has safety hazards such as causing cancer, weakened immunity, and triggering brain tumors during use. Although some sweeteners on the market have been replaced by aspartame and relatively safe sweeteners such as glucose syrup, their application is still very widespread, especially when used excessively, which may affect people's life safety.

AliTian is a dipeptide sweetener synthesized from aspartic acid and alanine as raw materials. Its sweetness is more than 2000 times that of sucrose, and it has the advantages of good safety, high sweetness, and low calorie content. It has stable properties and a wide range of applications. It is one of the substitutes for sucrose and has been approved for use in many countries, including China. Aspartame and Alisweet are commonly used sweeteners and are routine testing items in food testing.

This article uses PerkinElmer's new liquid chromatography LC 300 to simultaneously detect aspartame and alite in food, and establishes an accurate, reliable, and highly sensitive detection method.

This article uses PerkinElmer's LC 300 high-performance liquid chromatography to establish an analytical method for aspartame and alite in food. The results indicate that using Perkin Elmer LC 300 to analyze aspartame and alite has good sensitivity, excellent linearity and repeatability, meeting the quantitative requirements of this project.