AquaPenWorking principle of handheld algae fluorescence measuring instrument

AquaPen AP110Handheld algae fluorescence measuring instrument is a handheld fluorescence instrument used for rapid and accurate measurement of chlorophyll fluorescence parameters of aquatic algae and cyanobacteria.AquaPenThere are two types of probe models.AP110-CEquipped with a colorimetric cup test tube measurement room, collect the water, suspension or culture solution to be measured into the colorimetric cup for measurement455nmBlue and620nmLEDThe red light source can measure both chlorophyll fluorescence and measurement680nmand720nmOptical density.AP110-PEquipped with an immersion optical probe, it can be directly inserted into the water body, suspension or culture solution to be measured for measurement, as well as for measuring large algae.

AquaPenCapable of * sensitivity and detectable0.5Mg Chl/LChlorophyll fluorescence can detect natural water bodies with extremely low concentrations of phytoplankton, and can be used for field and laboratory measurements.

AquaPenAdopting debugging based fluorescence measurement technology, multiple parameters can be set to facilitate the measurement of chlorophyll fluorescence in various plants. Compact appearance, easy to carry, innovative design, simple operation, economical and durable, high precision and good stability.

Application field

·Study on the photosynthetic characteristics of algae and blue-green algae

·Algae content detection in water bodies

·Screening of photosynthetic mutants and phenotype research

·Detection of biotic and abiotic stress

·Study on the stress resistance or susceptibility of algae

·Economic algae breeding, disease detection, growth and yield evaluation

·teaching

Features:

§Compact structure and strong portability,LEDThe light source, detector, and control unit are integrated into a smartphone sized instrument, weighing only290g

§Powerful in function, it is a crystallization product of chlorophyll fluorescence technology, equipped with all the functions of a large fluorescence meter, and can measure all chlorophyll fluorescence parameters

§Built in all universal chlorophyll fluorescence analysis experimental programs, including two sets of fluorescence quenching analysis programs3Light response curve programOJIP–testwait

§High time resolution, achievable10Ten thousand times per second, automatically drawingOJIPCurve and provide26aOJIP–testparameter

§AquaPenTwo probe models: colorimetric cup test tube measurement chamber, which can measure both chlorophyll fluorescence and measurement680nmand720nmOptical density; Immersion optical probe, which can be directly inserted into the water body, suspension or culture solution to be measured for measurement, and can also measure large algae

§FluorPenProfessional software with powerful functions, can download and display chlorophyll fluorescence parameter charts, and can also directly control instruments for measurement through the software

§Equipped with unmanned automatic monitoring function

§Built in Bluetooth andUSBDual communication module,GPSModule, output chlorophyll fluorescence parameter chart with timestamp and geographic location

§Optional underwater automatic monitoring fluorescence meter, waterproof and dustproof design, depth10m

Measurement program and function

·FtInstantaneous chlorophyll fluorescence,After dark adaptation is completedFt＝F₀

·QYQuantum yield, representing the optical systemIIEfficiency is equal toFv/Fm(Dark adaptation state)orΦPSII(Light adaptation state).

·OJIPRapid Fluorescence Dynamics Curve, used to study the rapid fluorescence dynamics of plants after dark adaptation

·NPQFluorescence quenching kinetics curve, used to study the process of fluorescence quenching changes in plants from dark adaptation to light adaptation.

·LCLight response curve, used to study the fluorescence quenching response of plants to different light intensities.

·OD: Optical density, reflecting algal density(Limited)AP110-C）.

Technical Specifications

·Measurement parameters includeF₀TheFtTheFmTheFm’TheQYTheQY_LnTheQY_DnTheNPQTheQpTheRfdTheAreaTheMoTheSmThePITheABS/RCwait50Multiple chlorophyll fluorescence parameters,OD680andOD720(Limited)AP110-C）and3The light response curve of the light program3Fluorescence quenching curveOJIPCurve, etc

·OJIP–testThe time resolution is10µs(per second)10Ten thousand times), giveOJIPCurve and26A parameter, includingF₀TheFjTheFiTheFmTheFvTheVjTheViTheFm/F₀TheFv/F₀TheFv/FmTheMoTheAreaTheFix AreaTheSmTheSsTheNThePhi_PoThePsi_oThePhi_EoThePhi–DoThePhi_PavThePI_AbsTheABS/RCTheTRo/RCTheETo / RCTheDIo / RCwait

·Measurement program:FtTheQYTheOJIPTheNPQ1TheNPQ2TheNPQ3TheLC1TheLC2TheLC3TheOD680andOD720(Limited)AP110-C）TheMultiUnmanned automatic monitoring

·Measurement light: per measurement pulselight intensity0.09µmol(photons)/m².s，10-adjustable

·Photochemical light:10–1000µmol(photons)/m².sadjustable

·Saturated light:light intensity3000µmol(photons)/m².s，11-adjustable

·Probe model:AP110-CTest tube styleAP110-PProbe type

·light sourceAP110-C：620nmRed light and455nmBlue light measurement of chlorophyll fluorescence,680nmand720nmInfrared measurementOD；AP110-P：455nmblue light

·Test tube volume(Limited)AP110-C）：4ml

·Chlorophyll fluorescence detection limit:0.5Mg Chl/L

·Detector:PINphotodiode,667–750nmfilter

·Size: Ultra portable, phone size,165×65×55mm(excluding probe), weight only290g

·Data storage: capacity16Mb, can be stored149000data point

·Display and operation: graphical display, double key operation, standby mode5Minute automatic shutdown

·power supply2000mARechargeable lithium batteries,USBCharging, capable of continuous operation48Hour, low power alarm

·Working conditions:0–55℃，0–95%Relative humidity (without condensation)

·Storage conditions:-10–60℃，0–95%Relative humidity (without condensation)

·Communication method: Bluetooth+USBDual communication mode, Bluetooth in20mDistance transmission speed3Mbps

·GPSModule: Built in, precision1.5m

·softwareFluorPen1.1Specialized software for data download, analysis, chart display, and outputExcelData files and fluorescence kinetics curves, suitable forWindows 7And higher operating systems

Operating software and experimental results

AntarcticaMendelStation usageAquaPenChlorophyll fluorescence analyzer monitors the impact of Antarctic temperature rise on lichens/The impact of algae

Place of Origin:Europe

References

1. Zhang, C., Huang, X., Chu, Y., Ren, N. & Ho, S.-H. An overlooked effect induced by surface modification: different molecular response ofChlorella pyrenoidosato graphitized and oxidized nanodiamonds.Environ. Sci. : Nano10.1039.D0EN00444H (2020)

2. Arakaki, A.et al.Analysis of UV irradiation-induced cell settling of an oleaginous diatom, Fistulifera solaris, for efficient biomass recovery.Algal Research 47, 101834 (2020)

3. Contreras, J. A. & Gillard, J. T. F. Asparagine-based production of hydrogen peroxide triggers cell death in the diatomPhaeodactylum tricornutum. Botany Letters1–12 (2020)

4. You have to, L.et al.Bioprocess strategies for enhancing the outdoor production of Nannochloropsis gaditana: an evaluation of the effects of pH on culture performance in tubular photobioreactors.Bioprocess Biosyst Eng(2020)

5. Yaisamlee, C. & Sirikhachornkit, A. Characterization of Chlamydomonas Very High Light-tolerant Mutants for Enhanced Lipid Production.J. Oleo Sci. 69, 359–368 (2020)

6. Xu, M.et al.Co-culturing microalgae with endophytic bacteria increases nutrient removal efficiency for biogas purification.Bioresource Technology 314, 123766 (2020).

7. González-Camejo, J., Barat, R., Aguado, D. & Ferrer, J. Continuous 3-year outdoor operation of a flat-panel membrane photobioreactor to treat effluent from an anaerobic membrane bioreactor.Water Research 169, 115238 (2020).

8. Deng, X.et al.C*tion ofChlorella sorokinianausing wastewaters from different processing units of the silk industry for enhancing biomass production and nutrient removal.J Chem Technol Biotechnol 95, 264–273 (2020).

9. Tiwari, S., Verma, N., Prasad, S. M. & Singh, V. P. Cytokinin alleviates cypermethrin toxicity in Nostoc muscorum by involving nitric oxide: Regulation of exopolysaccharides secretion, PS II photochemistry and reactive oxygen species homeostasis.Chemosphere 259, 127356 (2020).

10. Wu, Y., Zhang, M., Li, Z., Xu, J. & Beardall, J. Differential Responses of Growth and Photochemical Performance of Marine Diatoms to Ocean Warming and High Light Irradiance.Photochem Photobiolphp.13268 (2020)

11. Abiusi, F., Wijffels, R. H. & Janssen, M. Doubling of microalgae productivity by oxygen balanced mixotrophy.ACS Sustainable Chemistry & Engineering 8, 6065–6074 (2020).

12. Rolton, A.et al.Early biomarker indicators of health in two commercially produced microalgal species im