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Shanghai Huxing Optical Instrument Co., Ltd
The use of eyepiece and objective lens
NegotiableUpdate on 12/11
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Overview
This article is organized and provided by an inverted microscope. Generally, an eyepiece with a moderate magnification (10) and a low magnification objective are used to observe, gradually switching to a higher magnification objective to find the magnification that meets the experimental requirements
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The use of eyepiece and objective lens
This article is organized and provided by an inverted microscope
This article is organized and provided by an inverted microscope
Usually, one is usedEyepiece with moderate magnification（10Start observing with a low magnification objective and gradually switch to a higher magnification objective to find the magnification that meets the experimental requirements.
When changing the objective lens, first observe with a low magnification lens and adjust to the correct working distance (clear imaging). If further using a high-power objective for observation, the part of the object image that needs to be magnified for observation should be moved to the center of the field of view before converting to a high-power objective (when converting a low-power objective to a high-power objective for observation, the range of the object image in the field of view is greatly reduced). The low-power objective lens and high-power objective lens are basically in focus (same high focus). When observing clearly with a low-power objective lens, changing to a high-power objective lens should allow the object image to be seen, but the object image may not be very clear. The fine focus screw can be rotated to adjust it.
It is generally believed that,When using any objective lensThe upper limit of effective magnification is1，000Multiplying by its numerical aperture, the lower limit is250Multiply its numerical aperture. in compliance with40What is the numerical aperture of the objective lens0.65The upper and lower limits are:1000×0.65=650Beihe and250×0.65≈163Exceeding the upper limit of effective magnification is called ineffective magnification, which cannot improve the observation effect. Magnification below the lower limit is difficult for the human eye to distinguish and is not conducive to observation. The general practical magnification range is500—700Multiply the number between numerical apertures.
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