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E-mail
root@apsmyx.com
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Phone
18001312670,17600971176
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Address
Room 821, No. 102 Chaoyang North Road, Chaoyang District, Beijing (100123)
Product Categories
Beijing Meiya Xian Technology Co., Ltd
DPatch patch clamp amplifier system
NegotiableUpdate on 09/24
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Overview
The patch clamp amplifier system consists of three components: patch clamp amplifier (including probe and preamplifier), digital to analog/digital converter, and acquisition and analysis software (including computer), commonly known as the "three piece set"
Product Details
The patch clamp amplifier system consists of three components: patch clamp amplifier (including probe and preamplifier), digital analog amplifier/Analog to digital converters, data acquisition and analysis software (including computers), commonly known as“three-piece set”.SutterThe company's patch clamp amplifier system (includingIPAanddPatchThe system is currently a complete three piece patch clamp system on the market.dPatchThe system includes: patch clamp amplifier (including probe and preamplifier, with single and double probe models), converter (notDendrite）AndSutterPatchSoftware.
dPatchApplication of the system
Low noise single channel recording
whole cell recording
Perforated patch clamp record
Voltage clamp and current clamp records
Recording of extracellular potentials in brain slices
intracellular recording
Lipid bilayer single channel recording
Single channel recording of liposomes
Recording of auditory and other rapidly changing signals
Study on the mechanism of cellular endocytosis/exocytosis (Lock In)
Research on Fast Power Clamp
Experimental research on attaching (cultivating) cells and cell lines
Experimental study on neural circuits in brain slices
In vivo patch clamp research
Research on Nanopores
Combining in vivo or ex vivo optogenetic research
Electrochemical (transmitter measurement) research
Amplifier parameters
1.1 Equipped with dual probes, equivalent to two patch clamp amplifiers. It also has a single probe patch clamp amplifier.
1.2 A highly automated amplifier controlled by a computer, with the instrument panel fully controlled by the computer, achieving the integration of the amplifier, converter, and software.
1.3 Equipped with a preamplifier, the signal is transmitted digitally instead of through traditional analog transmission, effectively reducing noise.
The 1.4 probe has capacitive feedback (Cf=1pF) and resistive feedback (Rf=50M Ω and 500M Ω) circuits, the former for single channel recording and the latter for whole cell recording.
The frequency bandwidth of the 1.5 probe input signal is: capacitor feedback circuit>500kHz, resistor feedback circuit>250kHz.
1.6 Due to the high resolution of the integrated analog-to-digital converter, there is no need to set amplifier output gain.
In the 1.7 voltage clamp mode, an 8-pole low-pass Bessel filter is used, and its open circuit noise level is as follows (RMS).
Capacitive feedback circuit (Cf=1pF): ≤ 25fA/1kHz, ≤ 95fA/5kHz, ≤ 220fA/10kHz
Resistance feedback circuit (Rf=500M Ω): ≤ 200fA/1kHz, ≤ 475fA/5kHz, ≤ 750fA/10kHz
Resistance feedback circuit (Rf=50M Ω): ≤ 650fA/1kHz, ≤ 1.5pA/5kHz, ≤ 2.4pA/10kHz
The maximum current recorded by the 1.8 probe is as follows.
Capacitive feedback circuit (Cf=1pF): ± 20nA, can record channel current within 1pA
Resistance feedback circuit (Rf=500M Ω): ± 20nA
Resistance feedback circuit (Rf=50M Ω): ± 200nA
1.9 has an 8-pole low-pass Bessel filter with a filtering frequency range of 100Hz~1MHz and more than 13 selectable bits.
1.10 has a voltage clamp mode, with a clamp potential output range of ± 750mV.
1.11 has a true current clamp mode (voltage follower):
The clamping current output range is ± 20nA (Rf=500M Ω) and ± 200nA (Rf=50M Ω)
Voltage clamp function with stable membrane potential in current clamp mode
Voltage rise time (Rs=10M Ω) ＜ 10 μ s
1.12 has automatic compensation function for electrode capacitance, membrane capacitance, and series resistance.
The maximum compensation amplitude value of electrode capacitance is 20pF, and the maximum compensation value of time constant is 10 μ s
The maximum compensation values for membrane capacitance are 500pF (Rf=500M Ω) and 1000pF (Rf=50M Ω)
The maximum compensation value for series resistance is 100M Ω (Rf=500M Ω) and 10M Ω (Rf=50M Ω)
1.13 has Lock In function (with a basic frequency of up to 20.8kHz), which can be used to study endocytosis/exocytosis mechanisms.
1.14 has a fast power clamp function, providing HH conductivity model, GHK permeability model, and Markov statistical model.
1.15 has an amplifier expansion panel, making communication with external devices easier.
1.16 Quartz electrode holder with ultra-low thermal expansion can effectively reduce electrode drift.
mathematical modeling/Parameters of analog-to-digital converter
2.1 Integrated with amplifier.
2.2 The resolution is 18-22 bits. When using a filtering frequency of 1kHz, the resolution can exceed 22 bits.
The maximum sampling frequency for a single lead is 5MHz.
2.4 Connect to the computer through a high-speed USB interface (3.0), which can be directly confirmed by the acquisition software.
2.5 Number of leads: There are a total of 8 analog signal inputs, 4 analog signal outputs, and 16 digital signal outputs.
2.6 Derivative input signal lead number: 10.
Data Acquisition and Analysis Software
3.1 Data collection and analysis are both conducted within the same software.
3.2 The acquisition software has the function of online clearing of 50Hz AC noise
3.3 The acquisition software has a Lock In function for measuring cell phagocytosis/exocytosis, with a measurement frequency of up to 1kHz.
3.4 The collected data can track and record up to 600 metadata describing experimental data (such as animal species, genotype, cell type, environmental temperature, resistance value, capacitance value, and other experimental related information).
The 3.5 analysis software has modules for neural discharge analysis, action potential analysis, camera image analysis, and single channel recording analysis.
3.6 The analysis software has a fast power clamp analysis function.
3.7 Analysis software can directly generate high-quality paper graphics for publishing articles.
3.8 Computer operating system: ≥ Windows 10 (64 bit), ≥ macOS 10.11 (El Capitan).
SutterPathsoftware
Based on Igor Pro 7 (WaveMetrics, Inc.)
Routines and Paradigms provide complete control over the experimental process
The waveform editor enables concise implementation of complex stimulus patterns or user-defined template editing
The associated metadata can store all information related to the experiment
Provide comprehensive data analysis programs and high-quality charts
Real time power frequency noise rapid response suppression
Can run on 64 bit systems such as Windows 7 and higher, or Macintosh OS X 10.11 (El Capitan)
configuration
dPatchApplication of the system
Low noise single channel recording
whole cell recording
Perforated patch clamp record
Voltage clamp and current clamp records
Recording of extracellular potentials in brain slices
intracellular recording
Lipid bilayer single channel recording
Single channel recording of liposomes
Recording of auditory and other rapidly changing signals
Study on the mechanism of cellular endocytosis/exocytosis (Lock In)
Research on Fast Power Clamp
Experimental research on attaching (cultivating) cells and cell lines
Experimental study on neural circuits in brain slices
In vivo patch clamp research
Research on Nanopores
Combining in vivo or ex vivo optogenetic research
Electrochemical (transmitter measurement) research
Amplifier parameters
1.1 Equipped with dual probes, equivalent to two patch clamp amplifiers. It also has a single probe patch clamp amplifier.
1.2 A highly automated amplifier controlled by a computer, with the instrument panel fully controlled by the computer, achieving the integration of the amplifier, converter, and software.
1.3 Equipped with a preamplifier, the signal is transmitted digitally instead of through traditional analog transmission, effectively reducing noise.
The 1.4 probe has capacitive feedback (Cf=1pF) and resistive feedback (Rf=50M Ω and 500M Ω) circuits, the former for single channel recording and the latter for whole cell recording.
The frequency bandwidth of the 1.5 probe input signal is: capacitor feedback circuit>500kHz, resistor feedback circuit>250kHz.
1.6 Due to the high resolution of the integrated analog-to-digital converter, there is no need to set amplifier output gain.
In the 1.7 voltage clamp mode, an 8-pole low-pass Bessel filter is used, and its open circuit noise level is as follows (RMS).
Capacitive feedback circuit (Cf=1pF): ≤ 25fA/1kHz, ≤ 95fA/5kHz, ≤ 220fA/10kHz
Resistance feedback circuit (Rf=500M Ω): ≤ 200fA/1kHz, ≤ 475fA/5kHz, ≤ 750fA/10kHz
Resistance feedback circuit (Rf=50M Ω): ≤ 650fA/1kHz, ≤ 1.5pA/5kHz, ≤ 2.4pA/10kHz
The maximum current recorded by the 1.8 probe is as follows.
Capacitive feedback circuit (Cf=1pF): ± 20nA, can record channel current within 1pA
Resistance feedback circuit (Rf=500M Ω): ± 20nA
Resistance feedback circuit (Rf=50M Ω): ± 200nA
1.9 has an 8-pole low-pass Bessel filter with a filtering frequency range of 100Hz~1MHz and more than 13 selectable bits.
1.10 has a voltage clamp mode, with a clamp potential output range of ± 750mV.
1.11 has a true current clamp mode (voltage follower):
The clamping current output range is ± 20nA (Rf=500M Ω) and ± 200nA (Rf=50M Ω)
Voltage clamp function with stable membrane potential in current clamp mode
Voltage rise time (Rs=10M Ω) ＜ 10 μ s
1.12 has automatic compensation function for electrode capacitance, membrane capacitance, and series resistance.
The maximum compensation amplitude value of electrode capacitance is 20pF, and the maximum compensation value of time constant is 10 μ s
The maximum compensation values for membrane capacitance are 500pF (Rf=500M Ω) and 1000pF (Rf=50M Ω)
The maximum compensation value for series resistance is 100M Ω (Rf=500M Ω) and 10M Ω (Rf=50M Ω)
1.13 has Lock In function (with a basic frequency of up to 20.8kHz), which can be used to study endocytosis/exocytosis mechanisms.
1.14 has a fast power clamp function, providing HH conductivity model, GHK permeability model, and Markov statistical model.
1.15 has an amplifier expansion panel, making communication with external devices easier.
1.16 Quartz electrode holder with ultra-low thermal expansion can effectively reduce electrode drift.
mathematical modeling/Parameters of analog-to-digital converter
2.1 Integrated with amplifier.
2.2 The resolution is 18-22 bits. When using a filtering frequency of 1kHz, the resolution can exceed 22 bits.
The maximum sampling frequency for a single lead is 5MHz.
2.4 Connect to the computer through a high-speed USB interface (3.0), which can be directly confirmed by the acquisition software.
2.5 Number of leads: There are a total of 8 analog signal inputs, 4 analog signal outputs, and 16 digital signal outputs.
2.6 Derivative input signal lead number: 10.
Data Acquisition and Analysis Software
3.1 Data collection and analysis are both conducted within the same software.
3.2 The acquisition software has the function of online clearing of 50Hz AC noise
3.3 The acquisition software has a Lock In function for measuring cell phagocytosis/exocytosis, with a measurement frequency of up to 1kHz.
3.4 The collected data can track and record up to 600 metadata describing experimental data (such as animal species, genotype, cell type, environmental temperature, resistance value, capacitance value, and other experimental related information).
The 3.5 analysis software has modules for neural discharge analysis, action potential analysis, camera image analysis, and single channel recording analysis.
3.6 The analysis software has a fast power clamp analysis function.
3.7 Analysis software can directly generate high-quality paper graphics for publishing articles.
3.8 Computer operating system: ≥ Windows 10 (64 bit), ≥ macOS 10.11 (El Capitan).
SutterPathsoftware
Based on Igor Pro 7 (WaveMetrics, Inc.)
Routines and Paradigms provide complete control over the experimental process
The waveform editor enables concise implementation of complex stimulus patterns or user-defined template editing
The associated metadata can store all information related to the experiment
Provide comprehensive data analysis programs and high-quality charts
Real time power frequency noise rapid response suppression
Can run on 64 bit systems such as Windows 7 and higher, or Macintosh OS X 10.11 (El Capitan)
configuration
| model | Product description |
| DPATCH | Digital patch clamp amplifier. Mainly includes: 1 probe, 1 preamplifier, 1 host, 1 EH-Q170 quartz electrode holder, 1 model cell, and SutterPatch software, etc |
| DPATCH-2 | Dual probe digital patch clamp amplifier. Mainly includes: 2 probes, 2 preamplifiers, 1 host, 2 EH-Q170 quartz electrode holders, 2 model cells, and SutterPatch software, etc |
| Possible spare parts (to be purchased separately) | |
| DPATCH-HS | DPatch probe and preamplifier |
| DPATCH-PCH | DPatch extension panel |
| EH-P170 | Polycarbonate electrode holder (suitable for 1.0-1.7mm outer diameter glass tubes) |
| EH-Q170 | Quartz electrode holder (suitable for 1.0-1.7mm outer diameter glass tubes) |
| GP-17 | Grounding copper rod |
