Amino acid detection in feed using liquid chromatography

according toMinistry of Agriculture announcesofLicense Conditions for Feed Enterprises and License Conditions for Mixed Feed Additive EnterprisesThe requirements for certificate replacement,companyAbsorb and digest the structural characteristics of international liquid chromatographs, and innovate independentlydevelopmentSpecializing inVitamins for the feed industry、amino acid、Melamine and other substances have established comprehensive detection methods and specialized configuration plans, providing feed enterprises withGMPCertification instrument configurationLC-3000IItypeeffectiveLiquid Chromatograph(Binary)high pressuregradientandComputer Anti ControlSincerely create value for users!The whole setThe instrument has good stability, simple operation, and easy maintenanceandComprehensive after-sales service can achieveNational regulated feedenterprisekarmaGMPauthentication.andWith its sensitivity, accuracy, and long-term provision of reliable and consistent performance stability, Luchuang's LC-3000II liquid chromatography instrument for amino acid detection in feed has become a leader among existing similar equipment. simultaneouslyThis series of liquid chromatographsCan be customized according to needsExpand various detectors, including UV, differential, fluorescence, and evaporative light scattering detectors, and can be configured with manual and automatic sampling systems，widely used infeedRaw material analysisAnalysis of Feed MixingChemical raw material analysis, pesticideswaitProcess control and other fields in enterprises.

Determination of feed content17Medium amino acidsvitaminAVitaminsD3VitaminsEFeed additives, streptomycin, ampicillin, trimethoprim, sulfaquinoxaline, etc.

1、 InstrumentMain performanceFeatures:

1LC-3000II liquid chromatography for amino acid detection in feedThe entire system is composed of P3000High pressure infusion pump (binary gradient), UV3000 spectrophotometerDetectorAnalytical dynamic high-pressure mixerInjection valve, solvent tray, chromatographic columnReverse control chromatographyworkstandSoftware and other components; Installation, debugging, and usage are all very simple.
2. The P3000 high-pressure infusion pump is a high-precision infusion pump system controlled by a microcontroller with CPLD technology as the core. The all digital frequency generation system, advanced motor drive system, and user-friendly human-machine display interface make operation very convenient. The advanced pump head and single valve design ideas integrate the world's best experience, ensuring the accuracy and repeatability of infusion under various conditions.

3. UV3000 is a spectrophotometer that can be matched with any infusion pump and is widely used in fields such as chemical analysis, pesticides, pharmaceuticals, and chemical engineering. Adopting a fully digital and highly integrated design concept, greatly reducing the noise of the instrument; Based on digital signal transmission and processing, the signal of this detector can be directly input into the serial port of the computer for acquisition, completely eliminating the data acquisition card used by traditional instruments!
2、 InstrumentMain technologiesparameter

·Infusion method: Double plunger series pump · Flow rate range: 0.001—9.999ml/min

·**Work pressure:42Mpa·Detection range:（190—700）nm, single wavelength

·Temperature control range: room temperature ·Response time: 0.01s

·Temperature compensation range: room temperature ℃·Pressure calibration range: * * 42000kPa

·Sampling frequency: unlimited·Resolution: 1024x768

·Control: panel control or workstation reverse control·Weight: 20kg

·Power: 460W·Dimensions: 398x360x460mm

·Measurement object: Amino acids in feedvitaminAnd detection of toxic and harmful substances

3、 Amino acid detection in feedLiquid ChromatographLC-3000IIThe three major technological advantages are:
1Extremely high sensitivity,≤2×10-9g/mlMethanol solution of naphthalene;
2Adopting electronic damping control（Electrical Dump Control）Effectively control flow pulsation through technology to ensure baseline noise;
3The dual path optical system can achieve dual wavelength detection.
4、 Analysis of chromatographic conditions for amino acid substances:

Sample:12Solution of non derived amino acid mixture（1mMol/L）

Chromatography instrument: "Lu ChuangLC-3000”Liquid chromatography, binary gradient

Equipped with diode array detector, evaporative light scattering detector, and chemical workstation

Detector:3000typeELSDEvaporative light scattering detector

Drift tube temperature is95℃, nitrogen flow rate2.5L/min

Chromatographic column:C18 5Mm, Column length625px, column diameter4.6mm

Mobile phase:ATo include0.1%TFAThe water

BTo include0.1%TFAAcetonitrile

gradient:time0min5min20min

Aliquid******60%

Bliquid0%0%40%

Current Speed:0.6mL/min

Sample injection:20ML

Result: All components are present25minAll components flow out from within, and each component is completely separated. The order of component flow out is: peak1For serine, peak2For lysine, peak3For glutamic acid, peak4For arginine, peak5For proline, peak6For valine, peak7For methionine, peak8For tyrosine, peak9For isoleucine, peak10For leucine, peak11For phenylalanine, peak12For histidine.

companyThe clothingtaskAdvantages:

1The technical indicators of our company's products strictly comply with the requirements of technical documents, and the product quality meets the requirements of relevant national standards.
2、The product is installed, debugged, and trained by technical personnel or provided with free telephone guidance.
3After the product is accepted as qualified,a yearDuring the warranty period, except for human damage factors, our company guarantees free maintenance；The equipment is maintained for life, and only cost fees are charged for vulnerable parts, while free technical support and discounted accessory supply are provided.
4The companyofafter-sales serviceEngineers have undergone chemical analysis、Training on instrument repair and maintenance，They can maintain and repair various components，To ensure that your instrument runs smoothly and quickly，Can guarantee to solve your problems quickly.
5、Timely telephone technical support----faultrowExcept for experts answering your calls，Provide timely solutions to problems.
6、****Service----distributionProvide high-quality services within the scope of * * * *.
7、flexiblefastResponse time----After phone repair report，1Provided within hourstimelyGuidance or72hourdoor-to-doorService.
8, uninterrupted24hourOnline Instrument Problem Answering.
9、There are free and irregular chromatography training courses every year.The content includes chromatographic principles、analyze、application、Technical instrument operation，helpAssist the causeObstacle removal and maintenance.

The company is one of the earliest manufacturers in China to start making gas chromatographs,Its productsExcellent performance、Reliable, it is a domestic chromatograph**The Influence ofStable brand. For decades, and salesLu Chuang'sbrandGCSeries gas chromatographs andLCSeries liquid chromatography instrumentSpread all over * * * *.