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Shenzhen Fiteli Technology Co., Ltd
Aspergillus flavus rapid analyzer
NegotiableUpdate on 05/14
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Overview
The Aspergillus flavus rapid analyzer FITLY-FQ, combined with the AflaQuik Aspergillus flavus total amount detection kit, can quickly and quantitatively detect the total content of Aspergillus flavus (B1, B2, G1, and G2) in various foods FDA certified
Product Details
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Aspergillus flavus rapid analyzer
Aspergillus flavus rapid analyzer
FITLY-FQ
The FITLY-FQ rapid detection instrument for Aspergillus flavus imported from the United States, combined with the AflaQuik Aspergillus flavus total amount detection kit, can quickly and quantitatively detect the total content of Aspergillus flavus (B1, B2, G1, and G2) in various foods FDA certified
The general agent of the Aspergillus flavus rapid analyzer imported from the United States in China; Shenzhen Fiteli Technology Co., Ltd
Instructions:
Aspergillus flavus is a naturally occurring bacterium that exists in many different foods. Foods contaminated with Aspergillus flavus can cause various diseases, such as liver cancer. The AflaQuik Aspergillus flavus Total Assay Kit includes an affinity chromatography column and reagents, making it convenient to extract, concentrate, and determine the total content of Aspergillus flavus (B1, B2, G1, and G2) from various food and feed samples. The AlfaQuik chromatography column contains solidified antibodies that can coagulate all Aspergillus flavus. When the food sample extract passes through the chromatographic column, Aspergillus flavus * * will adhere to the column, while other substances can pass through. Based on derivative reagents and Aspergillus flavus standards, purified samples can be directly measured at 360ex/460em using an Aspergillus flavus analyzer.
The Aspergillus flavus analyzer360ex/460emDirectly measure and purify the sample.
The general agent of the Aspergillus flavus rapid analyzer imported from the United States in China; Shenzhen Fiteli Technology Co., Ltd
Instructions:
Aspergillus flavus is a naturally occurring bacterium that exists in many different foods. Foods contaminated with Aspergillus flavus can cause various diseases, such as liver cancer. The AflaQuik Aspergillus flavus Total Assay Kit includes an affinity chromatography column and reagents, making it convenient to extract, concentrate, and determine the total content of Aspergillus flavus (B1, B2, G1, and G2) from various food and feed samples. The AlfaQuik chromatography column contains solidified antibodies that can coagulate all Aspergillus flavus. When the food sample extract passes through the chromatographic column, Aspergillus flavus * * will adhere to the column, while other substances can pass through. Based on derivative reagents and Aspergillus flavus standards, purified samples can be directly measured at 360ex/460em using an Aspergillus flavus analyzer.
The Aspergillus flavus analyzer360ex/460emDirectly measure and purify the sample.
【 Verification Effect 】
l Compared with enzyme-linked immunosorbent assay, it is fast, convenient, sensitive and**
l Aspergillus flavus rapid analyzerFITLY-FQ, scale * *, very portable
l Linear detection range:0.5 – 100 ppb
Note:Before calibration, place all components used for calibration at room temperature.
summary:AflaQuikThe total amount detection kit for Aspergillus flavus extracts Aspergillus flavus from food samples through a simple procedure and quickly determines its concentration using a fluorescence spectrophotometer. Pass the liquid extract of the sample through a chromatographic column to solidify Aspergillus flavus. After washing the chromatography column with cleaning solution, extract Aspergillus flavus with methanol. Mix the washing solution with derivative reagents to enhance the fluorescence of Aspergillus flavus, and then use a fluorescence spectrophotometer calibrated with Aspergillus flavus standard in the tool kit for measurement.
1. Extract (see attached table).
2. Color analysis (see attached table).
3. Translate into English50μLAdd the washing solution into a miniature glass tube. join50μLDilute the derivative reagent and mix it with a pipette3Next time. Prepare the 'sample' tube.
4. Standard formulation: Absorb100μL100ppbTransfer the standard sample of Aspergillus flavus to a miniature glass tube and prepare the "standard" tube. absorb100μLMethanol is used as a 'blank' tube.
5. Turn on the fluorescence spectrophotometer. Calibrate the reader,Place the 'blank' tube onto the sample rack.Press the "Calibration", "Calibration", and then the "Blank" button. The reader starts measuring. press“<- Std ->”Left arrow key, until the window displays“100.000”. Translate into EnglishStandard managementPlace it on the sample rack.Press' Measure '. The reader displays' Calibration completed '. The reader has been calibrated. Press the 'back' button.
6. Place the "sample" tube on the sample rack and follow the "measurement" instructions→Verification1”→'Measurement'. The concentration of Aspergillus flavus will be displayed in the window.Calculate the dilution factor for extracting and purifying Aspergillus flavus, and obtain the total concentration of Aspergillus flavus in the food sample. Record data or press the 'Save' button to save the data for retrieval. Press the 'back' button, and then Measure the next sample.
Attention: If the concentration of the "sample" exceeds the upper limit, dilute the sample in methanol and retest.
•AflaQuikVerification reagent kit: cat#:AflaQuik-25.Enough about25Secondary testing. Kit composition:（1）AlfaQuikChromatographic column（25ea）， （2）10Branched derivative reagent（3Milliliters), （3）Aspergillus flavus * * standard（15mL）.
Avoid contact or inhalation. When handling, laboratory safety standards and regulations should be followed, including wearing occupational safety and health regulations（OSHA）Approved protective goggles, gloves, and protective clothing.
Transportation and storage:The reagent kit should be transported refrigerated. The storage temperature of the tested substance is
•100 Miniature glass tube:cat# GTB-6x25.
•Aspergillus flavus rapid analyzerFITLY-FQThe reader is equipped with a5V/DC power adapter, oneUSBData cable, operation manual, and data management software disk.
Food Sample Preparation Procedure
Nuts (peanuts, cashews, walnuts, walnuts, almonds):
1. extract
l weighing
l and100Milliliters of methanol-Water mixture（60:40）Mixed.
l Mix at high speed for one minute. Pour the extract into folded filter paper and collect the filtrate in a clean container.
l Translate into English20Transfer milliliters of filtered extract to a clean test tube. use20Dilute with milliliters of deionized water and mix evenly. Filter the diluted extract into a clean test tube through a glass microfiber filter.
2. chromatography
l removeAflaQuikThe upper and lower covers of the chromatographic column. Discard the liquid from the top of the chromatographic column..
l Translate into English10milliliterDiluted filtered extractAt approximately per second1to2The dripping speed is completely transmitted through the chromatographic columnUntil the bubbles completely disappear.
l Translate into English10Milliliters of deionized water at a rate of approximately per second2The droplet velocity is passed through the chromatographic column and allowed to flow until the bubbles completely disappear.
l use2Wash the Aspergillus flavus * * in the chromatographic column with milliliters of high-performance liquid chromatography grade methanol into a clean test tube. The speed is approximately one drop per second.
Ginger powder, black pepper:
1. extract
l weighing
l and100Milliliters of methanol-Water mixture（80:20）Mixed.
l Mix at high speed for one minute. Pour the extract into folded filter paper and collect the filtrate in a clean container.
l Translate into English5milliliterFilteredTransfer the extract to a clean test tube. use20milliliter10%Twain20Dilute the solution and mix evenly. Filter the diluted extract into a clean test tube through a glass microfiber filter.
2. chromatography
l removeAflaQuikThe upper and lower covers of the chromatographic column. Discard the liquid from the top of the chromatographic column..
l Translate into English4Milliliters of filtered diluted extract at a rate of approximately per second1to2The dripping speed passes completely through the chromatographic column until the bubbles completely disappear.
l Translate into English10Milliliters of deionized water at a rate of approximately per second2The dripping speed passes through the chromatographic column.
l use2Wash the Aspergillus flavus * * in the chromatographic column with milliliters of high-performance liquid chromatography grade methanol into a clean test tube. The speed is approximately one drop per second.
Chili pepper, red pepper, paprika
（pepper Red Pepper Chili powder)
1. extract
l weighing
l and100Milliliters of methanol-Water mixture（80:20）Mixed.
l Mix at high speed for one minute. Pour the extract into folded filter paper and collect the filtrate in a clean container.
l Translate into English10Transfer milliliters of filtered extract to a clean test tube. use40Dilute with milliliters of deionized water and mix evenly. Filter the diluted extract into a clean test tube through a glass microfiber filter.
2. chromatography
l removeAflaQuikThe upper and lower covers of the chromatographic column. Discard the liquid from the top of the chromatographic column..
l Translate into English4Milliliters of filtered diluted extract at a rate of approximately per second1to2The dripping speed passes completely through the chromatographic column until the bubbles completely disappear.
l Translate into English10Milliliters of deionized water at a rate of approximately per second2The droplet velocity is passed through the chromatographic column and allowed to flow until the bubbles completely disappear.
l use2Wash the Aspergillus flavus * * in the chromatographic column with milliliters of high-performance liquid chromatography grade methanol into a clean test tube. The speed is approximately one drop per second.
Corn kernels, peanut butter:
1. extract
l weighing
l and100Milliliters of methanol-Water mixture（70:30）Mixed.
l Mix at high speed for two minutes. Pour the extract into folded filter paper and collect the filtrate in a clean container.
l Translate into English15Transfer milliliters of filtered extract to a clean test tube. use30Dilute with milliliters of deionized water and mix evenly. Filter the diluted extract into a clean test tube through a glass microfiber filter.
2. chromatography
l removeAflaQuikThe upper and lower covers of the chromatographic column. Discard the liquid from the top of the chromatographic column..
l Translate into English15Milliliters of filtered diluted extract at a rate of approximately per second1to2The dripping speed passes completely through the chromatographic column until the bubbles completely disappear.
l Translate into English10Milliliters of deionized water at a rate of approximately per second2The droplet velocity passes through the chromatographic column,Let it flowUntil the bubbles completely disappear.
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