Hygiena MCT-102 ATP fluorescence analyzer for water quality/food bacterial detection in the United States

Welcome to telephone consultation or online inquiry!
Hygiena MCT-102 ATP fluorescence analyzer for water quality/food bacterial detection in the United States
MCT-102
handheld

The Hygiena MCT-102 ATP fluorescence analyzer for water quality/food bacterial detection is a highly sensitive professional detection device designed to measure biological and chemical fluorescence intensity. It can be used alone or in conjunction with a computer through specialized software for operation.


Detection accuracy 1 X 10-18 mol/ATP/assay
The default detection time is 5 seconds (adjustable from 0.2-100 seconds)
Detection mode RLU, fg (bacterial cell ATP content)
The data storage results can be classified and stored, with 800 memories stored
Screen display LCD
Size/Weight 216 * 120 * 57 (length * width * height mm)/1.5 kg
Measurement value 0-3000000 RLU
**Error+/-5%
Real time detection and transmission of results when connected to a PC

ATP, short for adenosine triphosphate, is an intracellular energy supplying molecule. The MCT-102 multifunctional ATP rapid analyzer detects the luminescence reaction between ATP and luciferase in the sample, obtaining the light unit reading. After conversion, the total ATP level in the sample can be estimated, further indirectly determining the total number of microorganisms in the sample and providing reference for on-site detection of food hygiene supervision.
Firstly, use relevant reagents to degrade and eliminate ATP outside the bacteria. Reuse the extraction solution to release ATP from the bacteria and prevent its degradation through enzymes, followed by the addition of luciferase for luminescence reaction with ATP.
The luminescence value measured by a high-sensitivity photomultiplier tube is proportional to the actual ATP present. By comparing with the standard, it is possible to calculate the ATP content of the tested sample and express it in fg or RLU form.

【 Instructions for using the reagent kit 】
Instructions for using MCT-102 multifunctional ATP rapid analyzer kit
Microbial ATP Kit HS Biothema 266-112

1. The reagent kit includes the following reagents: (Each set of reagents can complete 100 experiments)
1.1. ATP Reagent HS (4 vials): Contains freeze-dried luciferase and luciferin. After participating in the reaction, luciferase consumes approximately 6% ATP per minute.
1.2. Diluent B 10ml (4 vials): Used as a buffer for reconfiguring ATP ReagentHS.
1.3. Extractant B/S 10 mL (1 small bottle): extractant.
1.4. ATP standard 5 mL (1 vial): ATP standard solution, containing 10-7 mol/L ATP.
1.5. ATP Eliminating Reagent (1 vial): ATP Eliminating Agent.
1.6. Cell Lysing Reagent 10 mL (1 vial): Cell lysing agent used for reconfiguring ATP Eliminating Reagent

2. Detection principle:
2.1. All living cells contain adenosine triphosphate (ATP) (molecular formula C10H16N5O13P3, chemical formula C10H8N4O2NH2 (OH) 2 (PO3H) 3H, molecular weight approximately 507.184)
2.2. The ATP content in a bacterium is about 2x10-18mol, and when the cell dies, ATP generally disappears as well. By detecting the ATP content in the sample, the total number of bacteria in the sample can be calculated.

3. Preparation before using reagents:
3.1. Uncover (do not use hands directly) the ATP Reagent HS bottle cap. Uncover the Diluent B bottle cap, pour Diluent B into the ATP Reagent HS reagent bottle, mix, and then pour the mixture back into the Diluent B reagent bottle. Store in the dark and refrigerated.
3.2. Extractant B/S and ATP Standard reagents can be used directly.
3.3. Mix ATP Eliminating Reagent with Cell Lysing Reagent for use. The mixture can be stored in a refrigerated state for more than a week, and in a frozen state for a longer period of time.

4. Detection reaction steps:
Firstly, ATP outside the bacteria is degraded by enzymes. Then use Extractant B/S to release intracellular ATP and prevent ATP degradation through enzymes. *Then add ATP Reagent HS to analyze ATP.
The ATP measured by a photometer is directly proportional to the actual ATP present. By measuring and comparing the results before and after adding a certain amount of standard ATP, it is possible to calculate the ATP content of the unknown sample being tested in the form of fg or RLU.

5. Testing steps:
1) Take the tested sample for pre-treatment according to international methods. (As explained later)
2) Take 50 µ L of processed sample and 50 µ L of ATP Eliminating Reagent in a test tube and let it stand for 10 minutes.
3) Add 50 µ L of Extractant B/S directly into the test tube, and then confirm that all ATP Eliminating Reagents have reacted with Extractant B/S (using a vortex mixer or manually shaking for a few seconds).
4) Add 0.4mL ATP Reagent HS and measure M1 Ismp.
5) Add 10 µ L of 100 nmol/L ATP Standard, approximately 1 pmol (10-12) ATP, and measure its value, M2 - Ismp+std。
Attention: The M2-M1: M1 value should be greater than 10, otherwise the sample needs to be diluted again for testing.
6) Calculate the total amount of ATP in the sample using this formula.

Product after-sales service
We have experienced technical engineers and professional management and marketing talents who can provide * suitable products and * professional services according to customer needs. Our service philosophy is: Fitly is dedicated to meeting your needs!
Hygiena MCT-102 ATP fluorescence analyzer for water quality/food bacterial detection in the United StatesWorth it! Welcome to call for consultation!

【 Related Information 】
ATP fluorescence analyzer PLUS
Hygiena MCT-102 ATP fluorescence analyzer for water quality/food bacterial detection in the United States
ATP fluorescence detector（Cleanliness tester
American Hygiena Ultrasnap ™ Integrated ATP detection swab
Hygiena snapshot from the United States ™ Universal ATP swab
American Hygiena Aquasnap ™ ATP water sample collection swab
Hygiena PRO Clean from the United States ™ Rapid protein residue detection swab


Regarding Fiteli
Shenzhen Fiteli Technology Co., LtdMain agency products:

Equipment for sample testing：

♦RBG's RVLM Microbial Rapid Detection System and RVL Rapid Detection Bottle from Germany
(Total number of live bacteria, coliforms, Escherichia coli, Enterobacteriaceae, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella, Listeria monocytogenes, Enterococcus, Clostridium sulfite reducing Clostridium, Clostridium perfringens, Aspergillus, Aspergillus, yeast, etc.)
♦ Swedish Meris MIRIS mid infrared dairy analyzer;
♦ Rapid detection instrument for total bacterial count in raw milk;
♦ Bostwick viscosity meter and moisture analyzer from CSC Corporation in the United States;
♦ Double U-shaped resonant intelligent liquid density meter/concentration meter/alcohol density meter;
♦ GoodJob series gel imaging products;
♦ Astori freezing point analyzer and colony counter from Italy;
♦ Organomation nitrogen blower from the United States;
♦ Japanese ATAGO refractometer/polarimeter;
♦ Palintest water quality analyzer from the UK;
♦ National Brick Products: Milk Microbial Activity Rapid Monitor, Grain Microbial Activity Rapid Monitor;
♦ Food sulfur analyzer (rapid detection of sulfur dioxide, sulfite, and suspended white blocks)
♦ Hygiena series products from the United States: SystemSURE Plus ATP fluorescence analyzer, EnSURE multifunctional ATP fluorescence analyzer
♦ And various food industry related testing instruments and consumables

Equipment used for sample processing:

♦ German ELMA ultrasonic cleaning machine;
♦ German KERN balance;
♦ ADAM balance in the UK
♦ Endecots screening machine and sieve from the UK
♦ Efficient tapping homogenizer/high-pressure homogenizer/ultra clean workbench/bacterial incubator from South Korean BNF company;
♦ SONICS ultrasonic disruptor from the United States.
♦ CENTURAON centrifuge from the UK;
♦ Italian PBI microbial sampler;
♦ AES Air Microbial Sampler, Beating Homogenizer, Automatic Dilution Device, and Culture Medium Automatic Preparation and Packaging System from France;

[Fiteli Recommended Products]
Microbial Rapid Detection System
ATP fluorescence analyzer
Ultrasonic cleaner
Infrared dairy analyzer
Food Safety Rapid Detector
Microbial Air Monitoring Systems