Blood cold storage is a storage warehouse used for storing blood. There are two types of blood cold storage: a whole blood cold storage (plasma cold storage) at 2-6 degrees Celsius, and a plasma freezer at -40 degrees Celsius.

Design requirements for blood cold storage

1. Blood cold storage should be equipped with dual power supply or emergency power generation equipment;

2. The warehouse should meet the requirements of cleanliness, hygiene, and grid power, and design work should be done well in the early stage of design; 3. Plasma and blood should be stored separately in dedicated blood cold storage; 4. Cold storage should be equipped with intelligent management, temperature and humidity recorders, sound and light alarm devices, etc.

The refrigeration temperature required for blood:

1. Whole blood and decellularized whole blood

1.1 Whole blood

1.1.1 Storage temperature: 2 ℃ to 6 ℃.

1.1.2 Shelf life: The shelf life of whole blood containing ACD-B and CPD blood preservation solutions is 21 days; the shelf life of whole blood containing CPDA-1 (adenine) blood preservation solution is 35 days. When using other blood preservation solutions, follow the shelf life specified in their instructions.

2.2 Removal of White Blood Cells from Whole Blood

2.2.1 Storage temperature: 2 ℃ to 6 ℃.

2.2.2 Shelf life: Same as 1.1.2.

2.2.3 White blood cells should be removed from whole blood within 48 hours after blood collection.

3 red blood cells

3.1 Concentrated red blood cells

3.1.1 Storage temperature: 2 ℃ to 6 ℃.

3.1.2 Shelf life: Same as 1.1.2.

3.2 Concentrated red blood cells after removing white blood cells

3.2.1 Storage temperature: 2 ℃ to 6 ℃.

3.2.2 Shelf life: Same as 1.1.2.

3.3 Suspended red blood cells

3.3.1 Storage temperature: 2 ℃ to 6 ℃.

3.3.2 Storage period: The storage period of suspended red blood cells in ACD-B and CPD red blood cell storage solutions is 21 days. The storage period of suspended red blood cells in CPDA-l or MAP red blood cell storage solutions is 35 days. The storage period of suspended red blood cells in 0.9% sodium chloride solution is 24 hours. When using other blood storage solutions, follow the storage period specified in their instructions.

3.4 Removal of white blood cells and suspension of red blood cells

3.4.1 Storage temperature: 2 ℃ to 6 ℃.

3.4.2 Shelf life: Same as 6.3.2.

3.5 Washing Red Blood Cells

3.5.1 Storage temperature: 2 ℃ to 6 ℃.

3.5.2 Shelf life: Add solution to 0 The storage period of washed red blood cells in a 9% sodium chloride solution is 24 hours. After washing in a closed system and suspending in red blood cell preservation solution, the storage period of washed red blood cells is the same as that of the red blood cell suspension before washing.

3.6 Frozen red blood cells

3.6.1 Storage temperature: Frozen red blood cells containing 20% glycerol should be stored below -120 ℃, and frozen red blood cells containing 40% glycerol should be stored below -65 ℃.

3.6.2 Shelf life: 10 years from the date of blood collection.

3.7 Freezing and thawing to remove glycerol red blood cells

3.7.1 Storage temperature: 2 ℃ to 6 ℃.

3.7.2 Shelf life: Add solution to 0 The shelf life of frozen thawed glycerol free red blood cells in a 9% sodium chloride solution is 24 hours. It is recommended to use frozen thawed glycerol free red blood cells as early as possible during the shelf life.

3.8 Special circumstances for storage period

When separating red blood cell components, if the closed system becomes an open system, the storage period is 24 hours, and it is recommended to use it as early as possible.

The blood bag used for blood collection [single (multiple) plastic blood bag] has the same shelf life as the stored blood after blood collection.

4 platelets

4.1 Concentrated platelets

4.1.1 Storage conditions: Store at a temperature of 20 ℃ to 24 ℃ and continuously shake gently.

4.1.2 Shelf life: When stored in a regular blood bag, the shelf life is 24 hours. When stored in a platelet specific blood bag, the shelf life is 5 days.

When a closed system becomes an open system, it should be stored for 6 hours and not exceed the original storage period.

When several concentrated platelets are collected in the same blood bag, traceability must be maintained, and the storage period after collection should be 6 hours, and not exceed the original storage period.

When there is no dedicated platelet storage equipment for continuous gentle shaking, the storage period is 24 hours and does not exceed the original storage period.

4.2 White blood cell concentration of platelets

4.2.1 Storage conditions: Store at a temperature of 20 ℃ to 24 ℃ and continuously shake gently.

4.2.2 Shelf life: Same as 3.1.2.

4.3 Single platelet collection

4.3.1 Storage conditions: Storage temperature is between 20 ℃ and 24 ℃. The well should be continuously shaken gently.

4.3.2 Shelf life: Same as 3.1.2.

4.4 Removal of White Blood Cells and Collection of Platelets

4.4.1 Storage conditions: Store at a temperature of 20 ℃ to 24 ℃ and continuously shake gently.

4.4.2 Shelf life: Same as 3.1.2.

4.5 Low plasma platelet count

4.5.1 Storage conditions: Store at a temperature of 20 ℃ to 24 ℃ and continuously shake gently.

4.5.2 Shelf life: Shelf life of 24 hours.

4.6 Wash platelets

4.6.1 Storage conditions: Store at a temperature of 20 ℃ to 24 ℃ and continuously shake gently.

4.6.2 Shelf life: Suspended at 0 The shelf life of 9% sodium chloride solution is 24 hours.

5 granulocytes

5.1 Storage temperature: 20 ℃~24 ℃.

5.2 Shelf life: The shelf life is 24 hours. It should be used after irradiation and should be used as early as possible.

6 Plasma

6.1 Fresh frozen plasma

6.1.1 Storage temperature: below -18 ℃

6.1.2 Shelf life: 1 year from the date of blood collection.

6.1.3 After thawing, it should be stored at 2 ℃ to 6 ℃ and infused within 24 hours.

6.2 Single collection of fresh frozen plasma

6.2.1 Storage temperature: below -18 ℃.

6.2.2 Shelf life: Same as 9.1.2.

6.2.3 After thawing, it should be stored at 2 ℃ to 6 ℃ and infused within 24 hours.

6.3 Frozen plasma

6.3.1 Storage temperature: below -18 ℃.

6.3.2 Shelf life: 4 years from the date of blood collection.

6.3.3 After thawing, it should be stored at 2 ℃ to 6 ℃ and infused within 24 hours.

6.4 Virus inactivation fresh frozen plasma (virus inactivation using methylene blue photochemical method) 6.4.1 Storage temperature: below -18 ℃.

6.4.2 Shelf life: Same as 9.1.2.

6.4.3 After thawing, it should be stored at 2 ℃ to 6 ℃ and infused within 24 hours.

6.5 Virus inactivation frozen plasma (virus inactivation using methylene blue photochemical method) 6.5.1 Storage temperature: below -18 ℃.

6.5.2 Shelf life: Same as 5.1.2.

6.5.3 After thawing, it should be stored at 2 ℃ to 6 ℃ and infused within 24 hours.

6.6 Cold precipitation coagulation factor

6.6.1 Storage temperature: below -18 ℃.

6.6.2 Shelf life: 1 year from the date of blood collection.

6.6.3 Early infusion is recommended after thawing. After thawing, it should be stored at 2 ℃ to 6 ℃ and infused within 24 hours. Thaw and infuse within 4 hours after mixing in an open system.

7 irradiated blood

7.1 Irradiated whole blood or irradiated red blood cell components

7.1.1 Storage temperature: 2 ℃ to 6 ℃.

7.1.2 Storage period: Whole blood and red blood cells should be irradiated within 14 days after collection, and the storage period after irradiation is 14 days.

7.2 Irradiated platelets

7.2.1 Storage conditions: Store at a temperature of 20 ℃ to 24 ℃ and continuously shake gently.

7.2.2 Storage period: The storage period after irradiation shall be the same as 3.1.2 and shall not exceed the original storage period.

7.3 Irradiated granulocytes

7.3.1 Storage conditions: 20 ℃ to 24 ℃.

7.3.2 Shelf life: Same as 4.2, and not exceeding the original shelf life.