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Phone
18584566129
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Address
4th Floor, Building 9, 4-1F, No. 2 Gang'an 2nd Road, Jiangbei District, Chongqing
Product Categories
Chongqing Zhongxian Optoelectronic Instrument Co., Ltd
Living Cell Imaging Environment Controller
NegotiableUpdate on 02/26
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Overview
The ZXMC-102 live cell imaging environment controller can be used in conjunction with a microscope for long-term delayed observation of cells. The controller includes a controller and a cell culture chamber, and can accurately control the temperature, carbon dioxide, and oxygen concentration of the cell culture chamber
Product Details
The ZXMC-102 live cell imaging environment controller can be used in conjunction with a microscope for long-term delayed observation of cells. The controller includes a controller and a cell culture chamber, and can accurately control the temperature, carbon dioxide, and oxygen concentration of the cell culture chamber. The cell observation chamber can be installed on commonly used inverted microscopes or on other cell microscopy imaging systems. Provide different size specifications to accommodate standard porous plates, culture dishes, and glass slides.
One applications
The cell observation chamber is used to place cell culture dishes or cell culture slides, and the built-in cells can be used for long-term observation of live cells (Time Lapse) experiments in a controlled environment (such as low oxygen environment).
II Product Features
Controllable parameters: temperature, oxygen concentration, carbon dioxide concentration
1. Oxygen concentration control range: 0 1% -25%, with an adjustment accuracy of ± 0 1%
2. Temperature control range: 30-40 ℃, with an accuracy of ± 0 1 ℃
3. Control range of carbon dioxide concentration: 0 1% -20%, with an adjustment accuracy of ± 0 1%
4. Controller size: 270 * 270 * 240mm
5. Cultivation chamber size: 202 * 127 * 39mm
III Model Description
Live Cell Station
----------------------------------------Operation steps---------------------------------------
1. Host: Connect the air inlet to the air source and adjust the pressure to 0.1Mpa. Connect the air outlet to the filter inlet, connect the filter outlet to the humidification bottle inlet, and connect the humidification bottle outlet to the observation cabin. Connect the observation cabin, microscope, and USB interface to the corresponding equipment, then turn on the power switch.
Note: After the host is powered on, please wait for 3 minutes to ensure that the sensor is fully preheated;
② The placement of the host must not be lower than the observation cabin to prevent liquid backflow in the water tank;
③ The pressure of the gas source must not exceed 0.1Mpa;
④ Pay attention to distinguishing the types of gases and do not connect them incorrectly;
⑤ The exhaust outlet of the host must not be blocked, as it may cause damage to internal components;
⑥ Single gas mode prohibits the use of humidification bottles;
⑦ Please add RO water or distilled water to the humidification bottle and replenish it once a day to prevent water shortage.
2. Observation cabin: Add 40ml of water to the sink, cover the sink lid, place an empty culture dish with the lid, connect the overflow port to a water bottle, and place the observation cabin on the observation cabin bracket.
Note: ① The observation cabin must not be placed at the air conditioning or fan vents, and direct sunlight is strictly prohibited;
② The circular hole on the observation cabin bracket must be located in the center of the culture dish;
③ The glass cover of the observation cabin is coated and must not come into contact with liquids other than water. UV irradiation can be used for disinfection;
④ The glass cover on the observation cabin is very thin, please handle it gently to prevent damage;
⑤ Observe the PC material of the compartment sink cover and prohibit high temperature and high pressure disinfection;
⑥ Please add RO water or distilled water to the observation cabin water tank and replenish it once a day to prevent water shortage;
⑦ Do not tilt or shake the observation cabin to prevent liquid from splashing into the interface and damaging the instrument;
⑧ The overflow bottle and water pipe must be below the height of the observation cabin.
3. Check whether the oxygen, carbon dioxide, observation cabin temperature, microscope temperature values, and observation cabin connection status are normal.
4. Set oxygen concentration, carbon dioxide concentration, gas control mode, and observation cabin temperature.
5. To set the temperature deviation of the observation cabin body and upper cover, please refer to the following table:
Note: The brand of the culture dish used for testing is "Beyotime". If the specifications or liquid level deviation of the culture dish causes temperature differences inside, please contact our after-sales guidance for debugging.
6. Click the "Start" button and run the instrument to preheat for 30 minutes.
7. Preheating is complete, replace the culture dish and start the experiment.
Note: Before placing the culture dish into the observation cabin, it is strictly prohibited for water droplets to condense inside the dish.
8. Remove the culture dish after the experiment is completed.
9. Click the "Stop" button, turn off the power, and unplug the communication cable and pipeline.
10. Use a syringe to extract the liquid from the sink to prevent it from splashing into the interface and damaging the instrument.
11. Clean the instrument.
----------------------------------------Calibration steps----------------------------------------
1. Unplug all gas pipelines from the host, turn on the air pump for ventilation for 15 minutes, and check if there is a significant deviation in oxygen concentration and carbon dioxide concentration.
Note: The oxygen concentration in the air is 20.9%, and the carbon dioxide concentration is 0.04%.
If there is a significant deviation, please contact our after-sales service engineer to obtain the calibration password and provide remote video communication guidance for calibration.
Note: Each calibration password is valid for 2 hours.
3. Remove all gas pipelines from the host and turn on the air pump to ventilate for 15 minutes.
4. Turn off the air pump and wait for 1 minute, then enter the calibration password to start calibration.
5. Calibration completed.
Live Cell Station
----------------------------------------Operation steps---------------------------------------
1. Host: Connect the air inlet to the air source and adjust the pressure to 0.1Mpa. Connect the air outlet to the filter inlet, connect the filter outlet to the humidification bottle inlet, and connect the humidification bottle outlet to the observation cabin. Connect the observation cabin, microscope, and USB interface to the corresponding equipment, then turn on the power switch.
Note: After the host is powered on, please wait for 3 minutes to ensure that the sensor is fully preheated;
② The placement of the host must not be lower than the observation cabin to prevent liquid backflow in the water tank;
③ The pressure of the gas source must not exceed 0.1Mpa;
④ Pay attention to distinguishing the types of gases and do not connect them incorrectly;
⑤ The exhaust outlet of the host must not be blocked, as it may cause damage to internal components;
⑥ Single gas mode prohibits the use of humidification bottles;
⑦ Please add RO water or distilled water to the humidification bottle and replenish it once a day to prevent water shortage.
2. Observation cabin: Add 40ml of water to the sink, cover the sink lid, place an empty culture dish with the lid, connect the overflow port to a water bottle, and place the observation cabin on the observation cabin bracket.
Note: ① The observation cabin must not be placed at the air conditioning or fan vents, and direct sunlight is strictly prohibited;
② The circular hole on the observation cabin bracket must be located in the center of the culture dish;
③ The glass cover of the observation cabin is coated and must not come into contact with liquids other than water. UV irradiation can be used for disinfection;
④ The glass cover on the observation cabin is very thin, please handle it gently to prevent damage;
⑤ Observe the PC material of the compartment sink cover and prohibit high temperature and high pressure disinfection;
⑥ Please add RO water or distilled water to the observation cabin water tank and replenish it once a day to prevent water shortage;
⑦ Do not tilt or shake the observation cabin to prevent liquid from splashing into the interface and damaging the instrument;
⑧ The overflow bottle and water pipe must be below the height of the observation cabin.
3. Check whether the oxygen, carbon dioxide, observation cabin temperature, microscope temperature values, and observation cabin connection status are normal.
4. Set oxygen concentration, carbon dioxide concentration, gas control mode, and observation cabin temperature.
5. To set the temperature deviation of the observation cabin body and upper cover, please refer to the following table:
| Specification of culture dish | φ35mm | Φ60mm | 6 holes | 12 holes | 24 holes | 48 holes | 96 holes |
| Liquid height inside the dish | 4.5mm | 5mm | 8.5mm | 8.5mm | 8.5mm | 8.5mm | 5mm |
| Body temperature deviation | 0℃ | 0℃ | 0℃ | 0.5℃ | 0.5℃ | 1℃ | 1℃ |
| Upper cover temperature deviation | 1℃ | 0.5℃ | 1℃ | 0.5℃ | 1℃ | 0.5℃ | 1℃ |
6. Click the "Start" button and run the instrument to preheat for 30 minutes.
7. Preheating is complete, replace the culture dish and start the experiment.
Note: Before placing the culture dish into the observation cabin, it is strictly prohibited for water droplets to condense inside the dish.
8. Remove the culture dish after the experiment is completed.
9. Click the "Stop" button, turn off the power, and unplug the communication cable and pipeline.
10. Use a syringe to extract the liquid from the sink to prevent it from splashing into the interface and damaging the instrument.
11. Clean the instrument.
----------------------------------------Calibration steps----------------------------------------
1. Unplug all gas pipelines from the host, turn on the air pump for ventilation for 15 minutes, and check if there is a significant deviation in oxygen concentration and carbon dioxide concentration.
Note: The oxygen concentration in the air is 20.9%, and the carbon dioxide concentration is 0.04%.
If there is a significant deviation, please contact our after-sales service engineer to obtain the calibration password and provide remote video communication guidance for calibration.
Note: Each calibration password is valid for 2 hours.
3. Remove all gas pipelines from the host and turn on the air pump to ventilate for 15 minutes.
4. Turn off the air pump and wait for 1 minute, then enter the calibration password to start calibration.
5. Calibration completed.
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