Preparation of full column imaging capillary isoelectric focusing electrophoresis iCIEF by CENitex

Preparation of full column imaging capillary isoelectric focusing electrophoresis iCIEF by CENitex

CEInfinite Preparation of Full Column Imaging Isoelectric Focusing Capillary Electrophoresis iCIEF

CEInfinite AutomatedPreparative iCIEF System

In response to the huge and urgent demand for identifying protein charge variants, AES company has developed the CEInfinite automated preparation iCIEF system.

• Excellent preparation performance: The proprietary reagent kit improves detection resolution, allowing even 0.1 pI differences to be distinguished and prepared. Typical automated operation takes less than 1 hour.

• High collection efficiency: The purity of collecting protein charge variants can reach over 90%, and a single run can collect 8 μ g of charge variants.

• Rapid method development: Directly convert methods from existing iCIEF platform methods.

• High reproducibility and efficiency:A fully automated collection system can be implemented100Mg/The amount of collection in the sky.

Details

Capillary variable diameter technology

CEInfinite has proprietary capillary diameter transfer technology (CDTT *) that allows for high-resolution preparation of charge change peaks. Once the protein is focused, the movement process begins, and the protein's charge variant is pushed out of the transfer capillary. This is visualized in real-time on the screen through iCIEF's Full Column Imaging Detection (WCID).

During the migration process, an electric field is still applied to the separation capillary. For high-purity fractions with low pI and different charge variants, CDTT maintains the separation resolution of the sample in a narrower transfer capillary.

4mg/ml NISTmAb， Preserved the overall resolution of proteins

By overlaying the iCIEF spectra of all collected fractions, the purity of the fractions can exceed 90%.