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Shenzhen Fiteli Technology Co., Ltd
Special pathogenic bacteria rapid detection system for the Administration for Industry and Commerce
NegotiableUpdate on 05/14
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Overview
The specialized pathogenic bacteria rapid detection system of the Administration for Industry and Commerce is dedicated to law enforcement by the Administration for Industry and Commerce. The instrument is easy to carry and can detect various types of food, drugs, water quality, object surfaces, and air. The instrument is a quantitative detection device that has been certified by the authoritative ISO 16140:2003 "Microbiology of Food and Animal Feed" alternative method, and meets the internal certification of ISO/IEC 17025:2005 standard. At present, the administration for industry and commerce in China has widely popularized the rapid quantitative detection of bacteria
Product Details
Welcome to telephone consultation or online inquiry!
FTL-RVLM specialized pathogenic bacteria rapid detection system for the Administration for Industry and Commerce
German RBG
FTL-RVLM
Measurement time: inversely proportional to bacterial content
Power supply: 220V
Dimensions: 300 * 200 * 100 (mm)
Weight: 2.5 (kg)
FTL-RVLM specialized pathogenic bacteria rapid detection system for the Administration for Industry and CommerceNo pre-treatment is required for sample testing
*Liquid samples, solid samples, and surface samples do not require pre-treatment
qualitative and quantitative analysis
*Paired with testing machine: 100% quantitative analysis
*Visual interpretation: qualitative or semi quantitative analysis
Simple and convenient
*Three simple steps can obtain the detection results
Quick inspection
*2-5 times faster than traditional inspection methods
High sensitivity
*Can detect 1 target microorganism (theoretical extremum)
high specificity
*Specificity up to 99.999% (theoretical extreme)
The testing bottle is the laboratory
*Microbial testing bottles are available at any time
*Testing personnel who have not received strict professional training can also conduct testing anytime and anywhere
Safely dispose of after use
*Handle the same as expired * *
[Scope of Application]
Combining the advantages of traditional inspection methods
【 No pre-processing required for sample inspection 】
Product after-sales service
We have experienced technical engineers and professional management and marketing talents who can provide * suitable products and * professional services according to customer needs. Our service philosophy is: Fitly is dedicated to meeting your needs!
Special pathogenic bacteria rapid detection system for the Administration for Industry and CommerceWorth it! Welcome to call for consultation!
【 Related Information 】
Microbial detection bottle
Yeast rapid detection bottle
Escherichia coli rapid detection bottle
Salmonella rapid detection bottle
FTL-RVLM specialized pathogenic bacteria rapid detection system for the Administration for Industry and Commerce
German RBG
FTL-RVLM
FTL-RVLM specialized pathogenic bacteria rapid detection system for the Administration for Industry and CommerceNo pre-treatment is required for sample testing
*Liquid samples, solid samples, and surface samples do not require pre-treatment
qualitative and quantitative analysis
*Paired with testing machine: 100% quantitative analysis
*Visual interpretation: qualitative or semi quantitative analysis
Simple and convenient
*Three simple steps can obtain the detection results
Quick inspection
*2-5 times faster than traditional inspection methods
High sensitivity
*Can detect 1 target microorganism (theoretical extremum)
high specificity
*Specificity up to 99.999% (theoretical extreme)
The testing bottle is the laboratory
*Microbial testing bottles are available at any time
*Testing personnel who have not received strict professional training can also conduct testing anytime and anywhere
Safely dispose of after use
*Handle the same as expired * *
【 Main Features 】
*1. Detection of foodborne pathogenic bacteria, total bacterial count, total mold count, etc
*2. Can detect solid, liquid, surface, paste, and slurry samples
*3. Instrument detection principle: ensemble culture dish method, enzyme method (β - glucosidase analysis), * * method (antigen search), and gene method (gene search)
*4. All 8 detection positions are independently operated to meet the needs of detecting different samples and microorganisms Each detection bit is independent
*To detect more samples, multiple machines can be connected to the same computer for use,
*6. It can automatically select and control the temperature of inspection items;
*7. The software is suitable for the JAVA environment.
*8. Internet connection, equipped with a 2.0 USB interface
*9. Simultaneous detection of three light waves (blue, green, red)
*10. Automatically generate quantitative analysis report after the detection is completed
*11. If the lid is folded during operation, the analysis will automatically stop, equipped with a light wave protection safety device.
*12. The sample does not require any pre-treatment process during detection and can be directly thrown into the detection bottle
*13. The detection sample only requires 1ml/1g;
*14. Sensitivity up to 1 target microorganism can be detected;
*15. The specificity is as high as 99.999%;
*16. Three simple operation steps, foolproof, no need for professional operators;
*17. The instrument is portable and can be used for detection and 100% quantitative analysis anytime and anywhere. It can also be directly connected to a computer to generate quantitative analysis reports.
*18. Passed the certification of ISO 16140:2003 "Microbiology of Food and Animal Feed" as an alternative to the authoritative certification, and complied with the internal certification of ISO/IEC 17025:2005 standard
[Scope of Detection]
→ Total number of live bacteria
→ coliform bacteria
→ Escherichia coli
→ Escherichia coli O157
→ Enterobacteriaceae
→ Staphylococcus aureus
→ Pseudomonas aeruginosa
→ Salmonella
→ Listeria
→ Enterococcus (Streptococcus)
→ Reduction of sulfite Clostridium difficile
→ Clostridium difficile
→ **Class (such as Aspergillus, etc.)
→ Yeast (such as yeast)
[Scope of Application]
Hygiene control:
-Food (HACCP)
-Kitchen, tools, surfaces (HACCP)
-Water quality
-(CDC) * * Control, Import and Export Inspection and Quarantine
-Medicines and cosmetics
It is closely related to our lives, for example:
Coffee shops, restaurants
Water source testing, water treatment plant
Analytical laboratory and HACCP diagnosis
Agricultural products and related processing companies
Pharmaceutical factories, pharmacies, cosmetics factories
Environmental monitoring agencies
水配送公司
Consumer protection groups, business management agencies
Indoor air conditioning regulation company
Combining the advantages of traditional inspection methods
-Petri dish method
-Enzymatic method (β - glucosidase analysis)
**Law (Antigen Search)
-Genetic method (gene search)
【 No pre-processing required for sample inspection 】
-Liquid samples, solid samples, and surface samples do not require pre-treatment
qualitative and quantitative analysis
-Paired with testing machine: 100% quantitative analysis
-Visual interpretation: qualitative or semi quantitative analysis
Simple and convenient
-Three simple steps to obtain detection results
Quick inspection
-2-5 times faster than traditional inspection methods
High sensitivity
-Can detect 1 target microorganism (theoretical extremum)
high specificity
-Specificity up to 99.999% (theoretical extreme)
The testing bottle is the laboratory
-Microbial testing bottles are available at any time
-Testing personnel who have not received strict professional training can also conduct testing anytime and anywhere
Safely dispose of after use
-Handle the same as expired * *
(Three simple steps to obtain 100% quantitative detection results)
**Step: Put in the sample
-Open the bottle cap and add 11ml of sterile water
-Add 0.1~1.0ml (0.1~1.0g) of liquid (solid/surface) sample
-Tighten the bottle cap tightly
**Step: Shake the bottle until the solvent is fully dissolved or mixed
(Fully dissolve or mix as shown in the figure on the right)
-If an oscillator is used, it takes about 20 seconds;
Or shake vigorously with your hands, which takes about 2-3 minutes.
Step 3: Read the results at an appropriate time (please refer to the table on the right)
-Using RVLM Microbial Detector:
-After fully dissolving and mixing, immediately put it into the detector
-Obtain 100% quantitative analysis results
If there is no RVLM microbiological detector and only RVL detection bottles are available:
-After fully dissolving and mixing, incubate in a thermostat
(Incubate at appropriate temperature for appropriate time, please refer to the table below)
-Visual interpretation of qualitative or semi quantitative analysis results
Taking Escherichia coli as an example:
If the bacterial count is greater than 108CFU/ml: The color turns yellow within 2 hours;
If the bacterial count is less than 102CFU/ml: The color will not turn yellow within 14 hours;
If the colony does not exist: the color still maintains its initial color after 18 hours
Or other intermediate colors (not turning yellow)
Aseptic treatment after use
-Press the top of the cap of the RVL microbiological detection bottle
-Safely dispose of with peace of mind (treated the same as expired * *)
Product Certification
FTL-RVLM specialized pathogenic bacteria rapid detection system for the Administration for Industry and CommerceHas been certified by the ISO 16140 alternative method
Adaptive verification
It should be noted that for food safety regulation, strict requirements are placed on the use of two analytical methods:
1) Food industry operators have a direct responsibility (automatic control) to regularly inspect food using third-party certification methods (according to HACCP procedures).
2) Certified laboratories (internal or external laboratories in the food industry) use reference methods or certified analytical methods to issue official testing reports on food.
The rapid microbial detection system RVLM is used for the above two analyses.
The committee standard issued on November 15, 2005
EC) N.2073/2005: Microbial standards for food
The fifth paragraph of Article 5: As long as it is recognized by the competent authority, food industry operators may use other sampling and inspection procedures. These steps may include sampling and trend analysis at different locations. The testing of non mainstream microorganisms and related microbial limits, including analytical testing, must comply with hygiene standards.
For example, the MBS method (Microbial Rapid Detection System RVLM) has been certified by ISO 13843 and can be used in food production processes (HACCP process), including food manufacturers, HACCP consulting companies, or certified laboratories.
The committee standard issued on November 15, 2005
EC) N.2073/2005: Microbial standards for food
The fifth paragraph of Article 5: Other analytical methods may be used after verification by the standard methods in Annex 1. If a brick method is certified by a third party according to the EN/ISO standard 16140 protocol or other internationally recognized protocols, it may also be used.
For example, the MBS method (Microbial Rapid Detection System RVLM) has been validated by reference methods and can be used for official product testing..
Published Confirmation Letter
G. Bottini, F. Losito, A. De Ascentis, F. R. Priolisi, A. Mari and G. Antonini
Confirmation of Microbial Testing Method for Total viable Bacteria and Escherichia coli in Food Samples
American Journal of Food Science and Technology, 6:951-962, 2011
DOI: 10.3923/ajft.2011.951.962 2011
F. Losito, G. Bottini, A. De Ascentis, F. R. Priolisi, A. Mari, G. Tarsitani and G. Antonini
Confirmation of quantitative and qualitative analysis of Listeria monocytogenes, Salmonella, Escherichia coli, and Staphylococcus aureus in food samples using MBS
American Journal of Food Science and Technology
Test applicability
Microbial rapid testing bottles can be used for internal automatic control of production processes and products.
Microbial rapid testing bottles can also be used to issue official testing reports and official hygiene inspections on food samples, similar to other analytical methods: 1) Laboratory (internal or external) certified for microbiological analysis; 2) The laboratory has demonstrated the ability to perform internal method testing in accordance with ISO/IEC 17025:2005 and correctly use the MBS method (§ 5.4.5.2 Non standard method testing).
In short, regardless of which analytical method is used, if the analysis result is positive, especially for specific microorganisms (such as Listeria monocytogenes), it is recommended to use other testing methods to verify and confirm the analysis.
In short, the RVLM microbiological detection system is your best choice Shenzhen Fiteli Technology Co., Ltd. is the general agent of RVLM microbial rapid detection system for Chinese enterprises, a general agent All staff will provide you with excellent pre-sales and after-sales service Service hotline: Contact person: Gao Xiaoling
Product Certification
FTL-RVLM specialized pathogenic bacteria rapid detection system for the Administration for Industry and CommerceHas been certified by the ISO 16140 alternative method
Adaptive verification
It should be noted that for food safety regulation, strict requirements are placed on the use of two analytical methods:
1) Food industry operators have a direct responsibility (automatic control) to regularly inspect food using third-party certification methods (according to HACCP procedures).
2) Certified laboratories (internal or external laboratories in the food industry) use reference methods or certified analytical methods to issue official testing reports on food.
The rapid microbial detection system RVLM is used for the above two analyses.
The committee standard issued on November 15, 2005
EC) N.2073/2005: Microbial standards for food
The fifth paragraph of Article 5: As long as it is recognized by the competent authority, food industry operators may use other sampling and inspection procedures. These steps may include sampling and trend analysis at different locations. The testing of non mainstream microorganisms and related microbial limits, including analytical testing, must comply with hygiene standards.
For example, the MBS method (Microbial Rapid Detection System RVLM) has been certified by ISO 13843 and can be used in food production processes (HACCP process), including food manufacturers, HACCP consulting companies, or certified laboratories.
The committee standard issued on November 15, 2005
EC) N.2073/2005: Microbial standards for food
The fifth paragraph of Article 5: Other analytical methods may be used after verification by the standard methods in Annex 1. If a brick method is certified by a third party according to the EN/ISO standard 16140 protocol or other internationally recognized protocols, it may also be used.
For example, the MBS method (Microbial Rapid Detection System RVLM) has been validated by reference methods and can be used for official product testing..
Published Confirmation Letter
G. Bottini, F. Losito, A. De Ascentis, F. R. Priolisi, A. Mari and G. Antonini
Confirmation of Microbial Testing Method for Total viable Bacteria and Escherichia coli in Food Samples
American Journal of Food Science and Technology, 6:951-962, 2011
DOI: 10.3923/ajft.2011.951.962 2011
F. Losito, G. Bottini, A. De Ascentis, F. R. Priolisi, A. Mari, G. Tarsitani and G. Antonini
Confirmation of quantitative and qualitative analysis of Listeria monocytogenes, Salmonella, Escherichia coli, and Staphylococcus aureus in food samples using MBS
American Journal of Food Science and Technology
Test applicability
Microbial rapid testing bottles can be used for internal automatic control of production processes and products.
Microbial rapid testing bottles can also be used to issue official testing reports and official hygiene inspections on food samples, similar to other analytical methods: 1) Laboratory (internal or external) certified for microbiological analysis; 2) The laboratory has demonstrated the ability to perform internal method testing in accordance with ISO/IEC 17025:2005 and correctly use the MBS method (§ 5.4.5.2 Non standard method testing).
In short, regardless of which analytical method is used, if the analysis result is positive, especially for specific microorganisms (such as Listeria monocytogenes), it is recommended to use other testing methods to verify and confirm the analysis.
In short, the RVLM microbiological detection system is your best choice Shenzhen Fiteli Technology Co., Ltd. is the general agent of RVLM microbial rapid detection system for Chinese enterprises, a general agent All staff will provide you with excellent pre-sales and after-sales service Service hotline: Contact person: Gao Xiaoling
Product Q&A
Microbial rapid detection bottle diagnosisRVL@productQ&A
Microbial rapid detection bottle diagnosisRVL@productQ&A
1. Can microbiological testing bottles detect dead bacteria in addition to live ones?
Answer: Cannot detect dead bacteria.
2. Can microbiological testing bottles detect * * *?
Answer: The detection range of the detection bottle includes * * * (Clostridium sulfite)Sulphite reducing Clostridia, Clostridium perfringensClostridium perfrigens）This also includes aerobic bacteria.
The reagents used in the detection bottles for detecting different types of microorganisms are different. For * * *, we have special reagents such as detecting Clostridium perfringensClostridium perfrigens.
3. What detection methods are used for the detection principles of detection bottles?
Answer: Culture dish method, enzyme method, * * method, gene method.
4. For the 3 Can you briefly describe the advantages and disadvantages of these methods?
Answer: Compared to traditional culture medium methods, colloidal gold, enzyme immunoassay, andPCRDifferent methods, the German * * microbiological detector is a collection of multiple methods. Using a single detection method has its own drawbacks: for example,
PCRThe law requires professional technicians and expensive equipment;
The operation of the cultivation medium method is complex;
The colloidal gold method has low sensitivity;
Insufficient specific capture by enzyme immunoassay.
The German * * Microbial Detection Bottle Rapid Detection Series products are a comprehensive application of multiple methods mentioned above, absorbing the advantages of each method and making up for the shortcomings of various methods.
5. When using a microbiological testing bottle for microbiological testing, should the sample or sterile water be added first?
Answer: Either is possible.
6. Do we need to inoculate samples with microorganisms in a sterile environment?
Answer: The detection bottle is analyzed by capturing specificity and combining multiple detection methods. Whether it is a sterile environment or not is acceptable (most of the testing sites are non sterile environments).
7. Can the detection bottle detect surface samples and solid samples?
Answer: Solid, liquid, and surface samples can be tested (by wetting the surface with sterile water using the provided cotton swab). Pulp and other paste like, paste like, and viscous substances can all be detected.
8. Do detection bottles need to undergo pre-treatment processes such as grinding and dilution when detecting solid samples?
Answer: Put the sample directly into the testing bottle without any pre-treatment.
9. What is the amount of sterile water used for each test?
Answer: Generally, it is11mlIf testing liquid or water samples, it is recommended to add samples1mlAdd sterile water10ml.
10. After adding the sample and sterile water,“Shake well to fully dissolve and mix”Is this operational step necessary for both qualitative and quantitative analysis?
Answer: Yes, shaking well is a necessary step for both qualitative and quantitative analysis.
11. Is the incubation temperature the same for different microorganisms?Please provide examples to illustrate.
Answer: It's different.37 Mostly in Celsius: total number of live bacteria, Salmonella, Listeria, etc., Escherichia coli 44 Celsius degrees, please refer to the comparison table for details.
12. If testing samples such as cold dishes or ice cream, should incubation be carried out at low temperatures?
Answer: There is no limit on the temperature of the test sample itself. For microorganisms in the test sample, please strictly follow the incubation temperature corresponding to the microorganisms being tested, and still refer to the control table.
13. If you want to speed up the detection time, can the incubation temperature be adjusted to be higher than the incubation temperature corresponding to the detected microorganisms?
Answer: No, strictly follow the comparison table.
14. What is the sample size specified in the operation manual?
Answer:0.1g‐1.0gor0.1ml‐1.0ml.
15. If the sample size is higher than the specified sample size, will the test results be different, or will the test results (when quantifying) increase?
Answer: If the sample size is larger or smaller, the quantitative analysis results will not change.
Reason: Traditional microbiological testing methods, including official reference methods such as colony counting based on solid selective culture media, have inherent statistical dispersion (statistical term, also known as statistical variation, is the spread of variables or probability distributions, common examples being variance, standard deviation, and interquartile range) greater than50%Many laboratories have confirmed that biological detection methods have lower statistical dispersion and higher reliability compared to other detection methods. But anyway,25‐30%There is still statistical dispersion. In addition, the statistical dispersion generated by sampling should also be considered
Solid meat products, especially those that are prone to microbial growth.
Therefore, adding samples is0.5gor1.5gThe results obtained are statistically equivalent (equivalent to the results obtained by any other method).
16. Can low-density bulk samples such as flour be tested as usual?
Answer: Sure.
17. Will dark samples like soy sauce affect the interpretation of qualitative testing results? Do you have any good suggestions?
Answer: If paired with a detector for quantitative analysis, there is no such problem; If qualitative analysis is only based on visual observation of discoloration, it is recommended to dilute dark samples before testing if there is concern that they may not be clearly recognizable by the naked eye. Do you still remember 15 Do you have the answer to the question? Adding samples is0.5gor1.5gThe results obtained are statistically equivalent to those obtained by any other method. The same principle applies to dilution.
18. If it is testing water samples, do we still need to add sterile water? How much to add?
Answer: It is recommended to add water samples1ml, sterile water10ml.
19. Are the starting colors and positive colors corresponding to different microorganisms the same? Please provide examples to illustrate.
Answer: Different.
For example, Salmonella incubation 10 The starting color that appears around minutes is red, and the positive color is yellow; Listeria monocytogenes starts with a blue color and a positive color is yellow. Please refer to the comparison table for details.
20Does the microbial detector also have the function of an incubation machine?
Answer: Yes. Shake the detection bottle thoroughly and immediately put it into the detector. After setting the detection bacteria on the software.
The detector will automatically incubate and provide a quantitative analysis report.
21. Does the microbiological detector also have the function of an oscillator?
Answer: Not available. Shake vigorously with your hands 2‐3 Minutes, or oscillator shake 20 Wait for about seconds until fully dissolved or mixed.
22. What is the reading principle of a microbiological detector?
Answer: Microbial detector is a precision optical reading instrument. Accurately detect color changes in the bottle through optical principles and provide a quantitative analysis and detection report.
23. Is the microbiological detector powered by an external source or comes with a rechargeable battery?
Answer: External power supply.
24. How many detection bottles can the microbial detector detect simultaneously and independently?
Answer:8 One. Simultaneously, independently detect and provide quantitative analysis reports separately.
25. Do I need to install analysis software on a computer for quantitative analysis with a microbiological detector?
Answer: It is necessary to follow the supporting analysis software.
26. What computer operating system is compatible with this analysis software?
Answer:XP, Vista, Windows 7
27. If the detector is powered on for the first time, how much time does it take to turn on the power before proceeding to the next step?
Answer: If the device is turned on for the first time, it will stay after turning on 40 It is advisable to continue operating in about seconds.
28. When conducting quantitative analysis with a detector, should the fully mixed detection bottle be immediately placed in the detector or wait for a moment before placing it in?
Answer: After thorough mixing, immediately put it into the detector.
29. After placing the detection bottle into the detector, click on "Start" in the analysis software of the detector. What color will the corresponding light for the detection hole change on the software interface?
Answer: Click on the operation interface corresponding to the detected bottle“Start”(Start) The indicator light for the corresponding hole changes from green to red. Indicates that the detection has started and is in progress.
30. After the detection is completed, the analysis software interface will restore the color of the corresponding hole's light, indicating that the detection is complete and a new sample can be placed?
Answer: During the inspection, the indicator light is red. After the detection is completed, the indicator light turns green. At this point, it is possible to place a new detection bottle into the corresponding detection hole of the detector to start the next round of testing. The detection time is determined by the presence of bacteria (when the microbial content is high), or by the corresponding detection time in the control table (when the microbial content is low, it should exceed the detection time in the control table)1CFUCorresponding detection time).
31. What is the relationship between the analysis time of microbial detection bottles and the microbial content in the detection bottles?
Answer: There is an inverse relationship. The higher the microbial content, the shorter the corresponding detection time; The lower the microbial content, the longer the corresponding detection time. If the microbial content in the sample is too high, the quantitative analysis results of the detector will significantly change within minutes or even seconds; If the microbial content is very low, (due to)RVLMIt is a very precise and sensitive instrument. Experienced use can preliminarily determine whether the target microbial content meets the specified requirements through changes in data over several hours or even minutes.
32. Should there be a clear experimental purpose before analyzing and testing to determine the microbial content you want to test (according to national standards for the amount of microorganisms present)?
Answer: This is * good.
For example, according to the countryGBStandard, or according to international standardsECThe standards clearly (or partially clearly) indicate the allowable maximum amount of microorganisms in different poultry and livestock species.
Specifically, when conducting microbiological testing, we must first clarify the purpose of the test. For example, internationallyEC 2073:2005According to the standard, the maximum allowable amount of Escherichia coli in fresh meat isCFU 10^2/gThe content of Escherichia coli in each gram of fresh meat is not allowed to exceedCFU 10^2At this point, based on the recruitment form, we find the column where Escherichia coli is located and checklog CFU 10^2=2Find the number in the Escherichia coli line 2 The corresponding number in column * * is 14Whether conducting qualitative analysis with the naked eye or quantitative analysis with a detector, it is necessary to check whether the content of Escherichia coli in the sample exceedsCFU 10^2, * growing in 14 Strict and accurate analysis results can be obtained within hours.
If quantitative analysis is conducted on the detection bottle, experienced experimenters will make a preliminary judgment on the microbial content in the detection bottle based on the dynamic changes in quantitative analysis data within a short period of time (such as ten minutes).
33. What is the purpose of clearly detecting microbial content, and what is the role of the reference table?
Answer: It is the basis for determining the incubation temperature, starting color, analysis time, and positive color standard control corresponding to the detected microorganisms. Please refer to the answer to the previous question for specific usage methods.
34. What isCFU?
Answer:colony‐forming unit,Colony forming unit.
The abbreviation for the unit of formation of bacterial clusters obtained through cultivation.
The units of measurement for bacteria (visible) and * *.CFU:colony‐forming unit,Colony forming unit refers to the process of diluting a certain amount of bacterial liquid by pouring or coating, allowing the individual microbial cells inside to disperse one by one on an agar plate. After cultivation, each living cell forms a colony. Unlike the conventional measurement of microbial count using a microscope, it is mainly a unit for measuring the number of visible (i.e., in most cases, forming colonies) bacteria. The meaning is how many single cells are contained in each milliliter of bacterial liquid!Traditionally, it is called“a”However, we know that a colony may not necessarily be generated by a single bacterium, but may also be generated by a cluster of bacteria (a cluster of bacteria)“a”It's not very accurate, the accurate name is“Colony forming unit”, English abbreviation“CFU”Just like“kilogram”and“kilogram”Just the name is different, there is no change in quantity.
CFUrepresent“colony forming units”.CFU/mLIt refers to the total number of bacterial communities contained in each milliliter of sample, which is also usefulCFU/gCorresponding to solid culture medium.
35. If I want to test the bacterial count of Escherichia coli in the sample, it must not exceed 106CFU/gor 106CFU/ml，
Observe the color changes in the bottle with the naked eye for qualitative analysis. Please compare“Incubation Temperature and Color Comparison Table”Elaborate on the testing steps.
Answer: * * Step: Insert the sample
Insert the sample to be tested（0.1‐1.0g, or 0.1‐1.0ml）Join in11mlAseptic water (based on the condition of the test substance, if it is a liquid, it is recommended to add a sample)1mlAdd sterile water10mlThe difference is not significant. Close the bottle cap tightly.
**Step: Shake the bottle until the solvent is fully dissolved or mixed.
If you need to shake vigorously with your hands 2‐3 About minutes; Or shake with an oscillator, it takes time 20 秒左右。
Step 3: Read the test results at the appropriate time
(1)If qualitatively analyzed with the naked eye. Determine the incubation temperature, starting color, detection time, positive color, and other information corresponding to the Escherichia coli population based on the reference table.
Incubation temperature: According to the control table 37℃；
Start color: Shake well and put in 37℃Incubation in a constant temperature incubator 10 The color will appear in about minutes. According to the comparison table, it is red;
Testing time: We need to test the standard that the coliform group content does not exceed 106CFU/gor 106CFU/mlAccording to the comparison table, corresponding columnslog 106=6Find the numbers 6 The corresponding row of numbers is 6The detection time is 6 Hour. That is, the observation time is determined as 6 Hour.
Positive color: According to the control table, the positive color for Escherichia coli is yellow.
After confirming the following information, we put the fully mixed detection bottle into it 37℃Keep the constant temperature box in this way. incubation 10 In about minutes, we will observe that the detection bottle will display the starting color corresponding to the detection of coliforms——red Continue to place, until 6 Observe for an hour, if the color turns yellow, it indicates that the coliform bacteria content in the sample exceeds 106CFU/gor 106CFU/mlThe sample is a non-conforming product; If it does not turn yellow but remains in its initial color of red or other intermediate colors, it indicates that the concentration of coliform bacteria in the sample does not exceed 106CFU/gor 106CFU/mlThe sample is a qualified product.
(2)If the detector performs quantitative analysis
Please refer to the next question for this step.
Step 4: Aseptic treatment
*Don't forget to press the top of the bottle cap to process the detection bottle. After pressing the bottle cap and shaking it, it is safe to discard.
Attention: When conducting experimental operations, do not touch the top of the cap of the test bottle to avoid improper aseptic treatment, which may affect the test results.
36. If 34 Please provide detailed steps for the quantitative analysis of * * using a testing device.
Answer:(2)If the detector performs quantitative analysis
Immediately place the fully mixed detection bottle into the detector.
Start the detector software and click“Station”(Status) Enter relevant information (including: inspector name, testing, customer to which the test sample belongs, testing time, etc.); In the software operation interface“analysis type”Select the microbial species to be detected from the dropdown menu and click on itokClick on start, the indicator light turns red,
Start entering the analysis state. Observe the quantitative analysis data changes of the detector and make judgments.
37. Why can't the cap of the test bottle be pressed before the end of the test?
Answer: Aseptic treatment. There are sterile substances in the bottle cap. Pressing the cap will allow the sterile substances in the cap to enter the bottle and react with the solvent inside, completing the sterile treatment. Therefore, please do not easily touch the cap of the detection bottle during the experiment.
Technicians are required to answer the following questions:
1. How many types of microorganisms can be detected by a detection bottle, and what are they?
Answer: Total number of live bacteria, coliforms, Escherichia coli, Enterobacteriaceae, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella, Listeria monocytogenes, Enterococcus, Clostridium sulfite reducing bacteria, Clostridium perfringens, Aspergillus, Aspergillus, yeast.
In the future, detection bottles for other microbial strains will be released one after another. Including Legionella bacteria, etc.
2. Can the detection bottle detect all strains of Escherichia coli (including the currently popular ones) when detecting Escherichia coliEHEC?）
Answer: Yes, all strains of Escherichia coli can be detected, includingEHECEnterohemorrhagic Escherichia coli（E.coli O104, O111, O157Etc.)
3. What are the application scope of detection bottles?
Answer: Hygiene control: Food(HACCP)Kitchen, tools, surfaces(HACCP), water quality,(CDC)**Control, import and export inspection and quarantine, drugs and cosmetics.
Related to our daily lives: environmental monitoring agencies, agricultural products and related processing companies, water source testing and water treatment plants, water distribution companies, pharmaceutical factories, pharmacies, cosmetics factories, analysis laboratories, andHACCPDiagnosis, dairy factories, cafes, restaurants, consumer protection groups, business management agencies, indoor air conditioning companies, especially suitable for rapid food testing in military and large-scale exhibitions.
4. Do you think that Escherichia coli detection bottles, Listeria monocytogenes detection bottles, and Salmonella detection bottles are not only used for food safety testing, but also suitable for medical purposes?
Answer: Escherichia coliEHECListeria monocytogenes and Salmonella, these three detection bottles can be used for in vitro clinical testing.
RoyalThe global positioning of the microbiological testing bottle series remains food safety. In Europe, clinical in vitro testing of human microbiota is very rare, and various aspects of safety control in Europe are well regulated. However, in developing countries, in vitro clinical testing of the three microorganisms mentioned above often occurs. The safety and accuracy of the above three detection bottles for in vitro clinical testing can be guaranteed.
4 combine, synthesize 1 The difference in market positioning between test strips and detection bottles
1）4 combine, synthesize 1 Comparison between test strips and detection bottles
4 combine, synthesize 1 The test strip, when combined with its analyzer, can be detected simultaneously by one analyzer 48 A test strip. Therefore, from the perspective of economic cost, * good startup is 48 Simultaneously inspect the film. For hospitals, save enough 48Analyze one fecal sample at a time.
Testing bottle, one sample can be used for inspection. And the bigger advantage is that this product will be very popular among doctors. Because doctors do not need to come into contact with samples, which is currently impossible for most hospital tests to achieve. Give the bottle to the patient and let them put in the stool sample themselves before sending it back to the doctor. The doctor takes the test bottle, shakes it evenly on the hospital's shaker, and places it in the incubator, waiting for the results to be interpreted.
2）Comparison between testing bottles and traditional testing methods currently used in hospitals
At present, the testing method in hospitals is still mostly the culture dish method. The thing that makes doctors dislike this method is that they have to come into contact with the sample. If a doctor is pregnant, the consequence of exposure to Listeria monocytogenes is miscarriage. If the testing station is not cleaned thoroughly, it can also easily lead to the spread of microorganisms.
Testing bottles, doctors do not need to touch the samples at all. The process of placing the sample into the testing bottle can be left to the patient to operate in the bathroom. What doctors need to do is simply shake well and incubate in an incubator (both oscillators and incubators are standard equipment in hospitals).
5. Whether it is solid, liquid, or surface samples, is there no need for pre-treatment during detection?
Answer: Yes
6. What is the specificity of the detector?
Answer:99.999%(Theoretical limit value)
7. What is the sensitivity of the detector?
Answer: It can be detected 1 The theoretical limit value of a living bacterium.
8. How many steps are required for qualitative analysis and what are they?
Answer: Three steps: sample addition; Shake the bottle until fully dissolved and mixed; Read the results at the appropriate time (according to the comparison table). *Don't forget to press the top of the bottle cap for aseptic treatment afterwards.
9. How many steps are required for quantitative analysis and what are they?
Answer: Same as above. Please refer to the operation manual of the detector for details.
10. What is the purpose of pressing the cap of the detection bottle after testing?
Answer: Aseptic treatment, please refer to the previous section 45 Question.
11. What is the storage temperature for the detection bottle?
Answer: In 20℃-25℃It can be stored on both sides and is convenient for transportation at this temperature, which is advantageous for temporary inspection. But with the greenhouse effect in the atmosphere, it is still important to pay attention not to exceed the storage temperature during summer 25℃Responsible for affecting the sensitivity of bottle detection. However, if it could be placed 4℃By storing, the shelf life can be extended by nearly twice.
12. What is the optimal storage temperature for extending the validity period of the test bottle?
Answer:4℃Please refer to the table below for details.
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