Ultra low diffusion chromatography (ULDC) with a pressure resistance of up to 862 bar

Ultra low diffusion chromatography (ULDC) with a pressure resistance of up to 862 bar

In the past few decades, in order to improve separation efficiency and shorten analysis time, the particle size of the stationary phase has been decreasing. When using these small-sized chromatographic columns, the liquid phase system often needs to withstand higher pressure resistance. After the introduction of UHPLC in 2004, the focus of competition among global chromatography manufacturers was to enhance the system's withstand voltage capability. The expensive high withstand voltage components led to high prices for UHPLC, limiting its popularity.

With the new AZURA 862 system and ultra-low diffusion chromatography (ULDC) system from Germany's KNAUER, let us enjoy the separation performance of UHPLC at the cost of HPLC.

AZURA 862beSpecially designed for high pressure requirements in chromatographic analysis. With a pressure resistance of up to 862 bar (12500 psi), it breaks the pressure limitations of traditional high-performance liquid chromatography systems and can be combined with small particle size chromatography columns to achieve largeSeparation ability.

Details

Almost all chromatography workers face problems with column effects and peak separation. In many cases, inappropriate column outer volume can cause chromatographic peak diffusion, disrupting the separation efficiency of HPLC, UHPLC, and chromatographic columns.

（Out of column effect: The flow path outside the chromatographic column from the injection system to the detector is affected by factors such as injection method and diffusion, resulting in a decrease in separation efficiency due to inappropriate out of column volume, as shown in the above figure, and even the inability to separate two components. ）

In the past few decades, in order to improve separation efficiency and shorten analysis time, the particle size of the stationary phase has been decreasing. When using these small-sized chromatographic columns, the liquid phase system often needs to withstand higher pressure resistance. After the introduction of UHPLC in 2004, the focus of competition among global chromatography manufacturers was to enhance the system's withstand voltage capability. The expensive high withstand voltage components led to high prices for UHPLC, limiting its popularity.

With the new AZURA 862 system and ultra-low diffusion chromatography (ULDC) system from Germany's KNAUER, let us enjoy the separation performance of UHPLC at the cost of HPLC.

AZURA 862beSpecially designed for high pressure requirements in chromatographic analysis. With a pressure resistance of up to 862 bar (12500 psi), it breaks the pressure limitations of traditional high-performance liquid chromatography systems and can be combined with small particle size chromatography columns to achieve largeSeparation ability.

Ultra Low Diffusion Chromatography (ULDC)

• Pressure resistance up to 862 bar (12500 psi)

• Total reflection technology is used to guide light through the pool, which has a small volume and high sensitivity

• Can be paired withChromatography column using fillers with particle size<2 μ m

• Ultra low diffusion volume, sharp peak shape, faster analysis speed

• The cost of HPLC benefits from the performance of UHPLC

• The traditional HPLC method can be directly transferred to ULDC without any adjustment.

Comparison of ULDC Separation Efficiency

Compared to conventional HPLC, the ULDC system with high pressure resistance (862 bar) uses finer pipelines and a total reflection technology flow cell with a smaller volume (2 μ l), which can ensure a smaller volume outside the column and a smaller effect outside the column. The chromatographic peaks are sharper, improving the sensitivity of the detection system.Due to its high pressure resistance, the ULDC system can also be paired with chromatography columns with particle sizes<2 μ m, allowing us to enjoy the performance of UHPLC at the cost of HPLC！